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Genome-wide maps of nascent transcriptions in proliferating Ctrl and Suv420h1 mutant muscle stem cells

ABSTRACT: To profile the nascent transcription activity including coding genes and eRNAs, we performed precision nuclear run-on sequencing (PRO-Seq) in control and Suv420h1 mutant MuSC

ORGANISM(S): Mus musculus

PROVIDER: GSE173257 | GEO | 2022/10/13

REPOSITORIES: GEO

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DRIP-seq mapping the R-loop formation in cultured Ctrl and Suv420h1 mutant MuSC

Project description:Inactivation of Suv420h1 in MuSC lead to increased transcription and replication collision thereby caused accumulation of RNA:DNA hybrids. This study profiles the R-loops in Ctrl and Suv420h1m mutant MuSCs.

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Cut & Run mapping the alteration of H4K20me1 and transcription activity in cultured MuSC upon Suv420h1 depletion.

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Genome-wide maps of nascent transcriptions in proliferating Ctrl and Suv420h1 mutant muscle stem cells

Project description:Genome-wide maps of nascent transcriptions in proliferating Ctrl and Suv420h1 mutant muscle stem cells

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DRIP-seq mapping the R-loop formation in cultured Ctrl and Suv420h1 mutant MuSC

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Project description:Here we report the cryo-electron microscopy (cryo-EM) structures of SUV420H1 associated with canonical nucleosome core particles (NCPs) or H2AZ containing NCPs. We find that SUV420H1 shows conformational change after bound to a nucleosome and make extensive site-specific contacts with histone and DNA regions, thus enabling H4K20 insertion for catalysis specifically. Through in vivo functional analysis, we validated special residues of SUV420H1 that are critical for its catalytic activity and function in chromatin state regulation.Thus, our study provides molecular insights into the nucleosome-based recognition and histone- methylation mechanisms of SUV420H1.

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