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Genes modulated by rhBMP2 and rhBMP7 during osteoblastic differentiation of C2C12 cells

ABSTRACT: This study analyses the gene expression profile of pre-osteoblastic C2C12 mice cells during osteodifferentiation in control and induced by rhBMP2 and rhBMP7 using DNA microarrays. Genes which were already known to be activated by BMP2, to be also activated by rhBMP7, indicating that BMP7 induces these genes in a similar fashion as BMP2. Moreover, we identified some new genes, such as Hoxc8, Glis1, Glis3, Ecsit, LrP6, Dvl1, Grn, BMP1, Ltbp2, Plod1, Htra1 and IGFBP-rP10, to be upregulated by both rhBMPs.

ORGANISM(S): Mus musculus

PROVIDER: GSE23783 | GEO | 2011/11/17

SECONDARY ACCESSION(S): PRJNA130723

REPOSITORIES: GEO

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Genes modulated by rhBMP2 and rhBMP7 during osteoblastic differentiation of C2C12 cells

Project description:This study analyses the gene expression profile of pre-osteoblastic C2C12 mice cells during osteodifferentiation in control and induced by rhBMP2 and rhBMP7 using DNA microarrays. Genes which were already known to be activated by BMP2, to be also activated by rhBMP7, indicating that BMP7 induces these genes in a similar fashion as BMP2. Moreover, we identified some new genes, such as Hoxc8, Glis1, Glis3, Ecsit, LrP6, Dvl1, Grn, BMP1, Ltbp2, Plod1, Htra1 and IGFBP-rP10, to be upregulated by both rhBMPs. Total RNA from mouse pre-myoblastic and pre-osteoblastic C2C12 cell line, after 12h of treatment with rhBMP2 and rhBMP7, was hybridized to a microarray and analyzed.

2011-11-17 | E-GEOD-23783 | biostudies-arrayexpress

Glis3: The role in osteoblast differentiation and adipocyte differentiation

Project description:The expression of Glis3 in C3H10T1/2 cells promotes osteoblastic differentiation as indicated by the the induction of increase in alkaline phosphatase activity, an early marker of osteoblast differentiation, and increased expression of osteopontin, a late marker of osteogenesis. Glis3 acts synergistically with bone morphogenic protein 2 (BMP-2). In contrast, expression of Glis3 inhibits the induction of adipocyte differentiation. Microarray analysis identified the fibroblast growth factor 18 (FGF18) as one of the genes induced by Glis3 in C3H10T1/2 cells directly. Keywords: Glis3, osteoblast differentiation, adipocyte differentiation, FGF18, BMP2

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Characterization of normal fibroblast transcriptomes upon transient silencing of LTBP2 by RNAseq

Project description:Purpose: Our single-cell RNAseq data show that LTBP2 is a marker of a cancer associated fibroblast subpopulation in colorectal cancer liver metastases. Moreover, MTT assays of cultured fibroblasts treated with anti-LTBP2 antibodies suggest that LTBP2 is essential for their survival. The goal of this study is to understand the role of LTBP2 in fibroblasts. Methods: Normal olon fibroblasts (CCD18Co) were transfected 48h with siRNA containing an anti-LTBP2 or a non target sequence. Cell monolayer total RNA of 3 biological replicates was then extracted and submitted to Illumina RNAseq using NovaSeq6000. Results: Transcriptomics revealed massive changes in gene expression when LTBP2 is knocked down: 496 genes were differentially expressed between the colon fibroblasts treated with siRNA anti-LTBP2 and NT. Those genes are implicated in diverse biological processes such as extracellular matrix organization, angiogenesis or cell adhesion. Conclusions: Our study explored LTBP2 functions in normal fibroblasts. RNAseq data support its important role. After 48h, LTBP2 silencing induces changes in gene expression of numerous genes involved in fibroblast functions.

2022-11-22 | GSE191323 | GEO

Dishevelled-1 regulates global transcriptomic changes in MDA-MB-231 triple negative breast cancer cells (RNA-Seq).

Project description:Dishevelled (DVL) is a crucial component of the Wnt-signaling pathway and is vital for multiple physiological processes. Previously thought to have a primarily cytoplasmic role, the discovery of DVL translocation to the nucleus reframed how DVL is viewed functionally. Despite our advances in elucidating DVL nuclear function, further investigation is needed to determine its transcriptomic and epigenetic regulatory abilities. We show here that modification of DVL1 expression globally affects the transcriptomic landscape. Additionally, analysis of DVL1 ChIP-seq allowed us to map global binding sites and reveal the extensive reach of DVL1 binding sites. Integration of RNA-Sequencing and ChIP-Sequencing further revealed DVL1 transcription factor binding partners to regulate gene expression. These findings provide insight to nuclear DVL1 regulation of gene transcription.

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Dishevelled-1 regulates global transcriptomic changes in MDA-MB-231 triple negative breast cancer cells (ChIP-Seq).

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RNAseq experiment to identify differentially expressed genes in dvl/rtfl mutants and DVL overexpresion lines in inflorescence apices of Arabidopsis thaliana

Project description:The lack of visible phenotypes of multiple dvl/rtfl mutants prompted us to compare the transcriptomic signatures of inflorescence apices of wildtype and mutant combinations (specifically dvl1 dvl8 dvl11 rtfl11 dvl19 and dvl8 dvl11 rtfl9 rtfl11 dvl19), as well as dvl1-D mutant, where DVL1 is overexpressed, and 35Spro:DVL8 line. Significant changes in gene expression were caused by the mutation of DVL genes in both quintuple mutant and overexpression backgrounds.

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Induction of insulin gene transcription by Gli-similar KrÃ¼ppel-like zinc finger protein 3, Glis3, in Î±TC1-9 cells

Project description:In this study, we examined the effect of Glis3 on the transcriptional activation of gene expression, including insulin gene, in pancreatic Î±-cell line, Î±TC1-9, which do not express the insulin gene. We demonstrate that Glis3 induces the transcription of the insulin and identified a number of other genes that are induced by Glis3. Using ChIP-Seq we map the genome-wide sites with which Glis3 is associated. From this the consesus Glis3 binding site was calculated. This study shows that Glis3 is recruited to the proximal promoter of the insuln gene inthe pancreatic Î±-cell line, Î±TC1-9.

2016-03-14 | E-GEOD-72566 | biostudies-arrayexpress

Regulation of gene expression by GLIS3 during neonatal kidney development

Project description:Loss of transcription factor GLIS3 function in humans and mice leads to the development of neonatal polycystic kidney disease (PKD). To investigate how loss of GLIS3 function in kidney affects postnatal kidney development and PKD, we analyzed the gene expression profiles of kidneys from WT and Glis3-null mice at P7, P14, and P28 by RNA-Seq analysis using Glis3 global KO model Glis3-mCherry and kidney specific knockout model Glis3-PAX8Cre.

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Examination of Glis3 binding and affects on gene expression in mouse pancreas

Project description:RNA sequencing of mouse islets from mice containing a pancreas-specific deletion of Glis3, and control littermates. Also, ChIP-seq analysis of Glis3 binding using a Glis3-eGFP mouse (endogenous Glis3 fused to enhanced GFP) and GFP antibody.

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iPS cell generation

Project description:This SuperSeries is composed of the following subset Series: GSE26428: Effect of Glis1 on human iPS cell generation GSE26429: Promotion of Direct Reprogramming by Glis1 GSE26430: Effect of Glis1, Dmrtb1, and Pitx2 on mouse iPS cell generation Refer to individual Series

2011-06-10 | E-GEOD-26431 | biostudies-arrayexpress

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