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Unknown,Transcriptomics,Genomics,Proteomics

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Induction of insulin gene transcription by Gli-similar KrÃ¼ppel-like zinc finger protein 3, Glis3, in Î±TC1-9 cells

ABSTRACT: In this study, we examined the effect of Glis3 on the transcriptional activation of gene expression, including insulin gene, in pancreatic Î±-cell line, Î±TC1-9, which do not express the insulin gene. We demonstrate that Glis3 induces the transcription of the insulin and identified a number of other genes that are induced by Glis3. Using ChIP-Seq we map the genome-wide sites with which Glis3 is associated. From this the consesus Glis3 binding site was calculated. This study shows that Glis3 is recruited to the proximal promoter of the insuln gene inthe pancreatic Î±-cell line, Î±TC1-9.

ORGANISM(S): Mus musculus

SUBMITTER: Raja Jothi

PROVIDER: E-GEOD-72566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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