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Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during the Development of Immunity

ABSTRACT: Afferent Lymph was collected over a 8 week period from up to 5 pen raised, nematode naïve, outbreed Romney sheep (Tag No’s 1001, 1002, 1003, 1004, 1006). During this time a Pre-Infection sample was collected, the sheep were immunised by 3 truncated (2 week) T. colubiformis infections (40,000 Tc L3, Immunising Infections 1-3). The treatment regime (shown below) will enable us to determine changes in gene expression during the development of immunity

ORGANISM(S): Ovis aries

PROVIDER: GSE23859 | GEO | 2010/09/01

SECONDARY ACCESSION(S): PRJNA133147

REPOSITORIES: GEO

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Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during the Development of Immunity

Project description:Afferent Lymph was collected over a 8 week period from up to 5 pen raised, nematode naïve, outbreed Romney sheep (Tag No’s 1001, 1002, 1003, 1004, 1006). During this time a Pre-Infection sample was collected, the sheep were immunised by 3 truncated (2 week) T. colubiformis infections (40,000 Tc L3, Immunising Infections 1-3). The treatment regime (shown below) will enable us to determine changes in gene expression during the development of immunity The overall design is summarised in the below table.

2010-09-01 | E-GEOD-23859 | biostudies-arrayexpress

Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during Challenge of Immunized Sheep

Project description:Afferent Lymph was collected from up to 5 pen raised, nematode naïve, outbreed Romney sheep (Tag No’s 1001, 1002, 1003, 1004, 1006) over a 12 week period. During this time a Pre-Infection sample was collected, the sheep were immunised by 3 truncated (2 week) T. colubiformis infections (40,000 Tc) followed by a Challenge Infection with 40,000 T. colubiformis L3 (Challenge Infection weeks 1-3). The Challenge Infection will enable us to determine changes in gene expression when the sheep gut rejects the parasitic nematodes.

2010-09-01 | GSE23863 | GEO

Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during Challenge of Immunized Sheep

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Characterization of host responses during Pseudomonas aeruginosa acute lung infection in the lungs and blood and after treatment with the synthetic immunomodulatory peptide IDR-1002

Project description:Pseudomonas aeruginosa is an opportunistic pathogen that causes nosocomial pneumonia and infects patients with cystic fibrosis. P. aeruginosa lung infections are difficult to treat due to bacterial resistance to antibiotics, and strains with multi-drug resistance are becoming more prevalent. Here we examined the use of a small host defense peptide, innate defense regulator 1002 (IDR-1002), in an acute P. aeruginosa lung infection in vivo. IDR-1002 significantly reduced the bacterial burden in the bronchoalveolar lavage fluid (BALF) as well as MCP-1 in the BALF and serum, KC in the serum, and IL-6 in the BALF. RNA-Seq was conducted on lungs and whole blood and the effects of P. aeruginosa, IDR-1002, or the combination of P. aeruginosa and IDR-1002 were evaluated. Differential gene expression analysis showed that P. aeruginosa increased multiple inflammatory and innate immune pathways as well as affected hemostasis, matrix metalloproteinases, collagen biosynthesis, and various metabolism pathways in the lungs and/or blood. Infected mice treated with IDR-1002 had significant changes in gene expression compared to untreated infected mice, with fewer differentially expressed genes associated with the inflammatory and innate immune responses to microbial infection, and treatment also affected morphogenesis, certain metabolic pathways, and lymphocyte activation. Overall, these results show that IDR-1002 was effective in treating P. aeruginosa acute lung infections and associated inflammation.

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Gene expression profile of germinal center B cells lacking Miz1 interaction with Myc

Project description:Splenic germinal center B cells (B220pos, CD19pos, CD38 low, FAS high) were isolated from animals 10 days sheep red blood cell immunised animals using flow cytometry cell sorter. Animals carry a combination of alleles that allows germinal center B cell deletion of endogenous Myc allele and simultaneous conditional overexpression of human MYC in its wild-type for or mutant form in which a valine in residue 394 has been substituted by an aspartic acid.

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Expression data of Hepatocellular Carcinoma

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Project description:Innate defense regulator (IDR) peptide-1002 is a synthetic host defense peptide derivative with strong anti-inflammatory properties.To investigate the anti-inflammatory mechanisms of IDR-1002 in vivo, the phorbol 12-myristate 13-acetate (PMA)-induced mouse ear inflammation model was used. Topical IDR-1002 treatment successfully dampened PMA-induced ear edema, proinflammatory cytokine production, reactive oxygen and nitrogen species release, and neutrophil recruitment in the ears of CD1 mice. Advanced RNA-Seq analysis on the mouse ear transcriptome revealed that IDR-1002 reduced sterile inflammation by suppressing the expression of transmembrane G-protein coupled receptors (Class A/1 rhodopsin-like), including receptors for chemokines, prostaglandins, histamine, platelet activating factor and anaphylatoxin. IDR-1002 also dampened IFNγ response and repressed the Interferon regulatory factor (Irf) 8-regulated network that controls central inflammatory pathways.

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Transcription profiling of chicken primary bronchus and secondary bronchi on birds vaccinated under various adjuvants and challenged by avian influenza virus H9N2.

Project description:Sylvia Reemers: chicken in vivo infection: timecourse, groupI= non immunised non challenged, groupIII= non immunised challenged, groupIV= immunised challenged, groupV= immunised+w/o challenged, groupVII= immunised+Al(OH)3 challenged, groupIX= immunised+CpG challenged Tissue type= Lung1. The aim of this study was to get a better understanding of the characteristics of a protective response and provide a signature of protection after vaccination with H9N2 adjuvanted with different immunomodulators.

2010-04-11 | E-MTAB-136 | biostudies-arrayexpress

Transcription profiling of chicken paleopulmonic parabronchi on birds vaccinated under various adjuvants and challenged by avian influenza virus H9N2.

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2010-04-01 | E-MTAB-137 | biostudies-arrayexpress

Transcription profiling of chicken trachea on birds vaccinated under various adjuvants and challenged by avian influenza virus H9N2.

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