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Gene expression profilling of poorly metastatic MDA cells and highly metastatic LM2 cells.

ABSTRACT: Comparison of gene expression between the breast cancer cell line MDA-MB-231 and its highly metastatic deriviate LM2 cells (described in Minn et al., Nature 2005). Results hilghlights potential metastasis promoting and suppressing genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE23904 | GEO | 2011/12/16

SECONDARY ACCESSION(S): PRJNA133185

REPOSITORIES: GEO

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Gene expression profilling of poorly metastatic MDA cells and highly metastatic LM2 cells.

Project description:Comparison of gene expression between the breast cancer cell line MDA-MB-231 and its highly metastatic deriviate LM2 cells (described in Minn et al., Nature 2005). Results hilghlights potential metastasis promoting and suppressing genes. 4 Samples

2011-12-16 | E-GEOD-23904 | biostudies-arrayexpress

miR-126 over-expression in highly metastatic LM2 breast cancer cells.

Project description:miR-126 were over-expressed using the miR-Vec system in highly metastatic LM2 cells. The LM2 cell line are described in detail in Minn et al. Nature 2005 This approach was used to conduct an unbiased search for specific miR-126 target genes in breast cancer cells.

2011-12-16 | GSE23905 | GEO

miR-126 over-expression in highly metastatic LM2 breast cancer cells.

2011-12-16 | E-GEOD-23905 | biostudies-arrayexpress

Gene expression profilling of poorly metastatic MDA cells and highly metastatic LM2 cells

| S-ECPF-GEOD-23904 | biostudies-other

Tumorigenic-enriched (TE) and lung-metastatic (LM) derivatives and parental populations from the breast cancer MDA-MB-231 and CN34 cell lines

Project description:The LM2 derivative cell line is described in Minn et al. Nature 2005. The LM1a derivative cell line is described in Tavazoie et al. Nature 2008.

2015-04-02 | GSE52629 | GEO

Tumorigenic-enriched (TE) and lung-metastatic (LM) derivatives and parental populations from the breast cancer MDA-MB-231 and CN34 cell lines

Project description:The LM2 derivative cell line is described in Minn et al. Nature 2005. The LM1a derivative cell line is described in Tavazoie et al. Nature 2008. Tumorigenic-enriched (TE) and lung-metastatic (LM) derivatives and their respective parental populations, from the breast cancer MDA-MB-231 and CN34 cell lines were transcriptomically compared in order to identify candidate regulators of breast cancer tumor re-initiation.

2015-04-02 | E-GEOD-52629 | biostudies-arrayexpress

RNA sequencing of MDA-LM2 and MDA-Parental cells

Project description:We used RNA sequencing to compare MDA-MB-231 cells to their higly metastatic MDA-LM2 derivatvies.

2021-03-30 | GSE160953 | GEO

Sequencing of human breast cancer cell line MDA-MB-231 and its highly pulmonary metastatic subline MDA-MB-231-LM2

Project description:Purpose: Tumor metastasis is the main cause of death from breast cancer patients and cell migration plays a critical role in metastasis. Recent studies have shown long non-coding RNAs (lncRNAs) play an essential role in the initiation and progression of cancer. In the present study, the role of a LncRNA, Rho GTPase Activating Protein 5- Antisense 1 (ARHGAP5-AS1) in breast cancer was investigated. Methods: RNA sequencing was performed to find out dysregulated LncRNAs in MDA-MB-231-LM2 cells. Transwell migration assays and F-actin staining were utilized to estimate cell migration ability. RNA pulldown assays and RNA immunoprecipitation were used to prove the interaction between ARHGAP5-AS1 and SMAD7. Western blot and immunofluorescence imaging were used to examine the protein levels. Dual luciferase reporter assays were performed to evaluate the activation of TGF-β signaling. Results: Compared to MDA-MB-231 cells, the expression of LncRNA ARHGAP5-AS1 (NR_027263) was significantly suppressed in its highly metastatic subtype MDA-MB-231-LM2 cells. Functional study showed ARHGAP5-AS1 could inhibit cell migration via suppression of stress fibers in breast cancer cell lines. Afterwards, SMAD7 was further identified to interact with ARHGAP5-AS1 by its PY motif and thus its ubiquitination and degradation was blocked due to reduced interaction with E3 ligase SMURF1 and SMURF2. Moreover, ARHGAP5-AS1 could inhibit TGF-β signaling pathway due to its inhibitory role on SMAD7. Conclusion: Overall, these findings demonstrate that ARHGAP5-AS1 inhibits breast cancer cell migration and could serve as a novel biomarker for breast cancer metastasis and a potent target for the treatment in the future.

2021-05-20 | GSE174717 | GEO

Next generation sequencing analysis of transcriptomes in MDA-MB-231 and LM2-4175 cell lines

Project description:LM2-4175 cell line was originally selected from MDA-MB-231,but has more aggressive characteristics in invasion, migration and metastasis. In addition, LM2 cell line specifically metastasizes to lung. To understand the melecular mechanisms of lung metastasis in breast cancer,we analyzed the RNA-seq data of MDA-MB-231 and LM2-4175 cell lines.

2019-07-31 | GSE124380 | GEO

Genome-wide profiles of multiple histone modifications in MDA-MB-231 and LM2-4175 cell lines

Project description:LM2-4175 cell line was originally selected from MDA-MB-231,but has more aggressive characteristics in invasion, migration and metastasis. In addition, LM2 cell line specifically metastasizes to lung. To understand the regulatory mechanisms of lung metastasis in breast cancer, we analyzed the chromatin structure of MDA-MB-231 and LM2-4175 cell lines.

2019-07-31 | GSE124379 | GEO

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