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Spaceflight-induced gene expression profiles in the mouse brain are attenuated by treatment with the antioxidant BuOE

ABSTRACT: The demands of deep-space pose a health risk to the central nervous system that has long been a concern when sending humans to space. While little is known about how spaceflight affects transcription spatially in the brain, understanding this has the potential to aid strategies that mitigate the effects of spaceflight on the brain. Therefore, we performed GeoMx Digital Spatial Profiling of mouse brains subjected to either space flight, or grounded controls. Four brain regions were selected: Cortex, Frontal Cortex, Corunu Ammonis I, and Dentate Gyrus. Antioxidants have emerged as a potential means of attenuating the effects of spaceflight, so we treated a subset of the mice with a superoxide dismutase mimic, MnTnBuOE-2-PyP 5+ (BuOE). Our analysis revealed hundreds of differentially expressed genes due to spaceflight in each of the four brain regions. Both common and region-specific transcriptomic responses were observed. Pathways sensitive to oxidative stress, and metabolic pathways, were enriched in the four brain regions due to spaceflight. These findings enhance our understanding of brain regional variation in susceptibility to spaceflight conditions. BuOE reduced the transcriptomic effects of spaceflight at a large number of genes, suggesting that this compound may attenuate oxidative stress-induced brain damage by the spaceflight environment.

ORGANISM(S): Mus musculus

PROVIDER: GSE239336 | GEO | 2023/09/13

REPOSITORIES: GEO

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Spaceflight-induced gene expression profiles in the mouse brain are attenuated by treatment with the antioxidant BuOE

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