Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The presence of Xanthine Dehydrogenase is crucial for the maturation of the rat kidneys

ABSTRACT: We hypothesized that XDH plays a role in nephrogenesis. Here we used SSXdh+/+, SSXdh+/-, and SSXdh-/- rat kidneys from the postnatal development period to test this hypothesis. In wildtype but not homozygote rat kidneys, XDH expression was significantly increased from postnatal day (PND) 5 to 15, indicating its importance in the maturation of the kidney. Even though both genotypes had similar glomerular density at birth, it was significantly lower in SSXdh-/- rats by PND21. Transcriptomic profiling of the kidneys revealed that many genes related to kidney formation were dysregulated by the lack of Xdh expression in this period. Functional and pathway analyses of the transcriptome data strongly suggested that Xdh affected these genes via epidermal growth factor (EGF) signaling disruption. This study showed that Xdh is essential for the kidney maturation process.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE239437 | GEO | 2023/09/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Lack of xanthine dehydrogenase leads to a remarkable renal decline in a novel hypouricemic rat model

Project description:The SSXdh-/- rat (SS-Xdhem1Mcwi, Rat Genome Database ID:14398479), created on the Dahl SS background, resulted in severe hypouricemia in the tissues. With the Xdh project, the overall hypothesis is that the Xdh gene ablation will result in kidney damage and perturbation of blood pressure control. We were interested in finding out if the gene ablation alternates the kidney development process. The deletion of Xdh protein produced dramatic changes in proteins related to the salvage pathway. The novel Xdh knockout rat model exhibited profound hypouricemia, which increased oxidative stress and crystal formation, leading to inflammation, renal cell proliferation, severe kidney damage, and a subsequent decline in renal function.

2022-03-17 | GSE198642 | GEO

Analysis of postnatal gene expression in a transgenic rat kidney

Project description:We have generated a transgenic rat model with postnatal pathology. In order to investigate the potential contribution of changes in kidney gene expression to the pathology, we have conducted microarray-based gene expression profiling of postnatal kidney. Rat kidneys were sampled at mid-day of postnatal day 6 for RNA extraction and hybridization to Affymetrix Genechip arrays. Two biological replicates of both wild-type and transgenic kidneys were included in the study.

2012-10-02 | E-GEOD-38595 | biostudies-arrayexpress

Effects of Developmental Lead Exposure on the Hippocampal Transcriptome.

Project description:In this study we analyzed the effects of lead-exposure up hippocampal gene expression in males and females exposed to 0ppm, 250ppm and 750ppm lead during two different developmental periods, perinatal (in utero through to weaning at PND21) and postnatal (PND0-PND45). All tissue was taken at PND 55. We used affymetrix Rat Gene 1.0ST arrays to obtain global gene expression data from each animal, with a group size of 4 for all conditions (Total number of Arrays = 40) Gene expression was profiled in hippocampus at no lead exposure (0ppm), 250ppm and 750 ppm lead exposure level at peinatal and postnaltal developmental period.

2012-05-30 | E-GEOD-38348 | biostudies-arrayexpress

Functional maturation of rat beta cells

2016-07-27 | GSE76969 | GEO

Functional maturation of rat beta cells

Project description:The weaning period consist of a critical postnatal window for structural and physiologic maturation of rat beta cells. To investigate transcriptome changes involved in the maturation of beta cells neighboring this period we performed microarray analysis in FACS beta cell enriched populations to detail the global programme of gene expression to identify its changes during this process. Male Wistar rats were selected at the initial point of a postnatal critical period (postnatal 20d) for pancreatic maturation for RNA extraction and hybridization on Affymetrix microarrays. We obtained immature and mature beta cell-enriched populations from postnatal 20d and 2 months animals in order to compare their expression profiles. To that end, we used primary cultured FACS-enriched beta cell populations according to their FSC, SSC and autoflourescence. We used three replicates for each condition.

2016-07-27 | E-GEOD-76969 | biostudies-arrayexpress

Molecular detection of maturation stages in the developing kidney

Project description:To identify maturation-dependent genes, we here performed single cell RNA sequencing (scRNA-seq) analysis using developing kidneys at different stages in the mouse, followed by highly sensitive in situ hybridization. We identified multiple genes expressed abundantly in newborn kidneys, but minimally at embryonic day 15.5. We then applied these maturation markers to the transplanted embryonic kidneys and found that the maturation process did not occur equally throughout nephron segments upon transplantation: glomeruli and proximal renal tubules became more mature than the other nephron segments. Thus, our scRNA-seq data at multiple stages of kidney development and identified maturation-dependent genes will serve as useful bases for assessing maturation of the developing kidney, and eventually of kidney organoids.

2020-11-19 | GSE149134 | GEO

rat kidney LC-MS - Exploring the Therapeutic Mechanism of Desmodium styracifolium on Oxalate Crystal-Induced Kidney Injuries Using Comprehensive Approaches Based on Proteomics and Network Pharmacology

Project description:Oxalate-induced kidney injury rat model were established.The differentially expressed proteins in the kidneys between the oxalate and control groups were explored.

2018-10-19 | PXD009496 | Pride

Genetic Diversity Influences the Response of the Brain to Developmental Lead Exposure.

Project description:In this study we analyzed the effects of lead-exposure up hippocampal gene expression in males and females exposed to 0ppm, 250ppm and 750ppm lead during two different developmental periods, perinatal (in utero through to weaning at PND21) and postnatal (PND0-PND45), across three strains (Fischer, Long Evans and Sprague Dawley). All tissue was taken at PND 55. We used affymetrix Rat Gene 1.0ST arrays to obtain global gene expression data from each animal, with a group size of 4 for all conditions (Total number of Arrays = 119) Gene expression was profiled in the hippocampus of rats at no lead exposure (0ppm), 250ppm and 750 ppm lead exposure levels during perinatal and postnatal developmental periods of both males and females of three strains of rat (Fischer, Long Evans and Sprague Dawley).

2014-04-10 | E-GEOD-56666 | biostudies-arrayexpress

The expression profiling of mouse kidneys during early developmental stages

Project description:Comparative analysis of gene expression patterns between prenatal (embryonic e16d) and postnatal (p1d and p7d) mouse kidney Total RNA was isolated of e16 kidney embryos, and kidneys from mice at posnatal day 1 and day 7

2011-09-30 | E-GEOD-28054 | biostudies-arrayexpress

Effects of Developmental Lead Exposure on the Hippocampal Transcriptome.

2012-05-31 | GSE38348 | GEO