Dataset Information

Rat kidney LC-MS - Exploring the Therapeutic Mechanism of Desmodium styracifolium on Oxalate Crystal-Induced Kidney Injuries Using Comprehensive Approaches Based on Proteomics and Network Pharmacology

ABSTRACT: Oxalate-induced kidney injury rat model were established.The differentially expressed proteins in the kidneys between the oxalate and control groups were explored.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Rattus Rattus (black Rat)

TISSUE(S): Kidney

DISEASE(S): Nephrolithiasis

SUBMITTER: Jiebin Hou

LAB HEAD: Xin Dong

PROVIDER: PXD009496 | Pride | 2018-10-19

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20161102_kidney_rat_8plex_iTRAQ_reviewed_PeptideSummary.xlsx	Xlsx
	20161102_kidney_rat_8plex_iTRAQ_reviewed_ProteinSummary.xlsx	Xlsx
	HLP-ITRAQ-F1.wiff	Wiff
	HLP-ITRAQ-F10.wiff	Wiff
	HLP-ITRAQ-F11.wiff	Wiff

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Publications

Exploring the Therapeutic Mechanism of Desmodium styracifolium on Oxalate Crystal-Induced Kidney Injuries Using Comprehensive Approaches Based on Proteomics and Network Pharmacology.

Hou Jiebin J Chen Wei W Lu Hongtao H Zhao Hongxia H Gao Songyan S Liu Wenrui W Dong Xin X Guo Zhiyong Z

Frontiers in pharmacology 20180613

Purpose: As a Chinese medicinal herb, Desmodium styracifolium (Osb.) Merr (DS) has been applied clinically to alleviate crystal-induced kidney injuries, but its effective components and their specific mechanisms still need further exploration. This research first combined the methods of network pharmacology and proteomics to explore the therapeutic protein targets of DS on oxalate crystal-induced kidney injuries to provide a reference for relevant clinical use. Methods: Oxal ...[more]

PMID: 29950996

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Project description:Kidney stones (KS) are very common, excruciating, and are associated with tremendous healthcare cost, chronic kidney disease (CKD), and end stage renal disease (ESRD). Most KS are composed of calcium oxalate and very small increases in urine oxalate concentration increase the risk for stone formation. Besides its critical role in the pathogenesis of KS, emerging data suggest that disturbed oxalate homeostasis (hyperoxaluria and/or hyperoxalemia) contributes to CKD progression, CKD - and ESRD-associated cardiovascular diseases, progression of cyst growth in autosomal dominant polycystic kidney disease (ADPKD), and delayed graft function & poor renal allograft survival. This emphasizes the urgent need for plasma and urinary oxalate lowering therapies, and enhancing the bowel’s ability to secrete oxalate may effectively do so. We previously identified Oxalobacter formigenes (O. formigenes)-derived factors secreted in its culture conditioned medium (CM) which stimulated oxalate transport by human intestinal Caco2-BBE (C2) cells and reduced urinary oxalate excretion in hyperoxaluric mice by enhancing colonic oxalate secretion. Given their remarkable therapeutic potential, we now identified several proteins belonging to Sel1-like family as the major O. formigenes-derived secreted factors, and we determined the crystal structures for six proteins to better understand their function. Importantly, Sel1-14-derived small peptides P8 & P9 were identified as the major factors, with P8+9 closely recapitulate the CM’s effects, including acting through the oxalate transporters SLC26A2 & SLC26A6 and PKA activation. P8+9 also stimulate oxalate transport by human ileal and colonic organoids, confirming that these peptides work in human tissues. Collectively, the identification of these small peptides provide a great opportunity for developing a peptide-based novel therapeutic for hyperoxalemia, hyperoxaluria, and related disorders, impacting the outcomes of patients suffering from KS, primary hyperoxaluria, CKD, ADPKD, ESRD, and renal transplant recipients.

Dataset Information

Rat kidney LC-MS - Exploring the Therapeutic Mechanism of Desmodium styracifolium on Oxalate Crystal-Induced Kidney Injuries Using Comprehensive Approaches Based on Proteomics and Network Pharmacology

Dataset's files

Publications

Exploring the Therapeutic Mechanism of <i>Desmodium styracifolium</i> on Oxalate Crystal-Induced Kidney Injuries Using Comprehensive Approaches Based on Proteomics and Network Pharmacology.

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