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LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice

ABSTRACT: This study revealed the heterogeneity of UC-MSCs using single-cell sequencing technology (10x Genomics). Through in vitro and in vivo experiments, it was determined that the LRP1high UC-MSCs subpopulation has therapeutic effects in improving ovarian function. This finding provides theoretical support for the screening of stem cell preparations for use in POF, aiming to reduce the impact of other interfering cell components on ovarian tissue and provide precise, safe, and effective clinical strategies for stem cell therapy in POF. Mechanistic exploration further revealed that the LRP1high UC-MSCs subpopulation primarily improves mitochondrial function of granulosa cells by modulating DNA damage pathways, extracellular matrix-related signals, and cellular metabolism. Consequently, it more effectively enhances the quality of aging mouse oocytes, offering new insights into targeting LRP1high UC-MSCs for precise treatment of premature ovarian failure.

ORGANISM(S): Mus musculus

PROVIDER: GSE239500 | GEO | 2024/03/13

REPOSITORIES: GEO

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LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice.

Project description:This study revealed the heterogeneity of UC-MSCs using single-cell sequencing technology (10x Genomics). Through in vitro and in vivo experiments, it was determined that the LRP1high UC-MSCs subpopulation has therapeutic effects in improving ovarian function. This finding provides theoretical support for the screening of stem cell preparations for use in POF, aiming to reduce the impact of other interfering cell components on ovarian tissue and provide precise, safe, and effective clinical strategies for stem cell therapy in POF. Mechanistic exploration further revealed that the LRP1high UC-MSCs subpopulation primarily improves mitochondrial function of granulosa cells by modulating DNA damage pathways, extracellular matrix-related signals, and cellular metabolism. Consequently, it more effectively enhances the quality of aging mouse oocytes, offering new insights into targeting LRP1high UC-MSCs for precise treatment of premature ovarian failure.

2024-03-13 | GSE240185 | GEO

LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice.

Project description:LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice.

| PRJNA1002589 | ENA

LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice

Project description:LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice

| PRJNA999596 | ENA

POF (MO 459).D.mel 3rd Instar Larvae Nuclei POF Mutant

Project description:modENCODE_submission_3794 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. The proteins under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: POF mutant Developmental Stage: 3rd Instar Larvae; Genotype: POF [D119]/POF [D119]; Transgene: deletion; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Strain POF mutant Antibody POF (MO 459) (target is POF); Developmental Stage 3rd Instar Larvae

2013-02-22 | E-GEOD-44496 | biostudies-arrayexpress

Pof D119 mutant expression analysis in Drosophila melanogaster testis

Project description:To study the influence of POF on transcription in testis we made expression arrays from dissected testes (Pof mutants and wild type control). It is clear that POF mainly alters the expression of genes on the fourth chromosome Keywords: mutant analysis

2009-03-30 | GSE14516 | GEO

Pof D119 mutant expression analysis

Project description:Analysis of gene expression in pof deletion mutants. Chromosome 4 genes are down-regulated in pof mutants compared to wildtype control. 200 Drosophila melanogaster first instar larvae were used for each of three biological replicates of y1 w67c23; PofD119/PofD119 and three biological replicates of y1 w67c23 as controls. Keywords: Mutant vs Wt expression

2007-03-22 | GSE6839 | GEO

Pof mutant expression analysis

Project description:Analysis of expression in pof mutant and wt 1st instar larvae Keywords: Mutant vs Wt expression

2007-03-22 | GSE5984 | GEO

NanoSTRING dendritic cell subpopulation

Project description:NanoSTRING dendritic cell subpopulation

| PRJNA975120 | ENA

Serum Exosomal Omics Analysis: Revealing Energy Metabolism Anomalies and Promising Diagnostic Markers for Premature Ovarian Failure

Project description:Background: Premature ovarian failure (POF) is a clinical condition characterized by the cessation of ovarian function, leading to infertility. The underlying molecular mechanisms remain unclear, and no predictable biomarkers have been identified. This study aimed to investigate the protein and metabolite contents of serum exosomes to identify underlying molecular mechanisms and explore potential biomarkers. Methods: This study was conducted on a cohort consisting of 14 POF patients and 16 healthy controls. The exosomes extracted from the serum of each group were subjected to label-free proteomic and unbiased metabolomic analysis. Differentially expressed proteins and metabolites were annotated. Pathway network clustering was conducted with further correlation analysis. The biomarkers were confirmed by ROC analysis and random forest machine learning. Results: The proteomic and metabolomic profiles of POF patients and healthy controls were compared. Two subgroups of POF patients, Pre-POF and Pro-POF, were identified based on the proteomic profile, while all patients displayed a distinguishable metabolomic profile. Signaling pathway clustering revealed progression of dysfunctional energy metabolism during the development of POF. The differentially expressed proteins and metabolites were highly correlated, with six of them selected as potential biomarkers. ROC curve analysis and random forest machine learning suggested that AFM combined with GE was a diagnostic biomarker for POF. Conclusion: Omic analysis revealed that inflammation and oxidative stress are factors that damage ovarian tissue, but progressive dysfunction of energy metabolism might be the critical pathogenetic pathway contributing to the development of POF. AFM combined with GE together serves as the best biomarker for clinical POF diagnosis.

2024-10-17 | PXD051569 | Pride

Chromatin IP of POF, HP1 and H3K9ac in S2 cells and salivary glands.

Project description:Two chromosome-targeting and gene regulatory systems are present in Drosophila melanogaster. The male X-chromosome is targeted by the male-specific lethal complex believed to mediate the two-fold up-regulation of the X-linked genes. The highly heterochromatic 4th chromosome is specifically targeted by the Painting of Fourth (POF) protein which together with heterochromatin protein 1 (HP1) modulate the expression level of genes on the 4th chromosome. Here we report high-resolution mapping of POF and HP1 on the 4th chromosome using chromatin immunoprecipitation followed by tiling microarrays (ChIP-chip) in S2 cells and salivary glands. The enrichments are compared to transcript profiles of the two cell types used. POF specifically binds to genes with a strong preference for exons. The POF binding profile correlates to the binding profile of HP1 which in addition displays a typical “peak” in the promoter regions of bound genes. POF and HP1 binds to active genes and the binding correlates with levels of transcription and changes in binding levels are paralleled by a comparable change in transcription. Our results provide a high resolution description of the chromosome 4 specific gene regulatory system provided by the combinatorial effects of POF and HP1. Keywords: ChIP-chip

2007-10-03 | GSE8296 | GEO

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