Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Genetic Evidence for the Gatekeeping Role of Ybx1 in Controlling Follicle Activation and Early Folliculogenesis by Regulation of Cdkn1a in the Zebrafish

ABSTRACT: Y box-binding protein 1 (YB-1; Ybx1/ybx1) regulates transcription and translation of targeted genes by DNA/RNA-binding. Our previous proteomic study demonstrated a dramatic drop in Ybx1 protein during follicle activation in zebrafish ovary. In this study, we created an ybx1 mutant with CRISPR/Cas9, and showed that the folliculogenesis in the mutant ovary (ybx1-/-) was blocked at pre-vitellogenic (PV)-to-early vitellogenic (EV) transition, leading to small ovaries. The mutant females were therefore sub-fertile with reduced fecundity. RNA-seq and Western blot analyses identified a variety of genes that showed differential expression between mutant ovary (ybx1-/-) and the control (ybx1+/-) including cdkn1a (p21). Disruption of cdkn1a resulted in embryonic lethality. In p21 heterozygotes (cdkn1a+/-), however, follicle activation and maturation in the ovary were both enhanced in contrast to ybx1 mutant (ybx1-/-). Interestingly, partial loss of p21 in heterozygotes (cdkn1a+/-) could rescue the phenotype of ybx1 mutant (ybx1-/-). Folliculogenesis resumed in ybx1-/-;p21+/- females with normal follicle activation, in contrast to the PV-EV blockade in ybx1-/- mutant. Interestingly, the follicle cells from the ybx1-/- mutant follicles displayed a poor proliferative activity in vitro; however, the cells from the ybx1-/-p21+/- follicles resumed normal proliferation compared to that from the wildtype fish. In summary, we demonstrated in this study that Ybx1 played a gatekeeping role in controlling PV-to-EV transition and it might act by suppressing the expression of cdkn1a, a cell cycle inhibitor.

ORGANISM(S): Danio rerio

PROVIDER: GSE239623 | GEO | 2024/10/27

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Oogenesis in Solea senegalensis: Previtellogenic versus vitellogenic ovaries

Project description:Transcriptionnal profile changes in ovary during the reproductive cycle of Solea senegalensis: from previtellogenic stage to vitellogenic stage obtained at different times during the annual reproductive cycle. An oligo microarray based on ESTs sequenced from S. senegalensis previously designed (CerdÃ et al., 2008, BMC genomics) was used. The two stages of ovary development were directly compared using three individuals for each stage (IR-Pv-1, IR-Pv-2, IR-Pv-3 for previtellogenic individuals and IR-Vt-1, IR-Vt-2, IR-Vt-3 for vitellogenic individuals). For the experiment, the vitellogenic stage was used as the control.

2010-06-21 | E-GEOD-17336 | biostudies-arrayexpress

CDKN1A Deficiency in Zebrafish Promote Follicle Maturation and Degeneration Mediated by CRISPR/Cas9 Targeted Disruption

Project description:CDKN1A plays multiple roles in distinctive biological processes, such as cellular proliferation, apoptosis, DNA repairing, and etc. In mouse, Cdkn1a deficiency led to cell arrest at G1 phase, and also reported that Cdkna1-deficient mice were susceptible to tumorigenesis. However, the functions of Cdkn1a in zebrafish were still elusive due to few studies. Our previous study indicated that Cdkn1a might be closely associated with folliculogenesis, and to further investigate its specific role during folliculogenesis, Cdkn1a was targeted disrupted by CRISPR/Cas9. Interestingly, Cdkn1a-deficient zebrafish were embryonic lethality, resulted in approximate 2% homozygous mutants in adult stage. Furthermore, homozygous mutants have accelerated follicle maturation that faster than WT, and were fertile at young age (2 month). However, the matured or large follicles in mutant soon become dysfunctional due to abnormal oocyte that led to infertile at 3 months. Gradually, the abnormal follicles were undertaken follicle degeneration, resulted in PG follicle enrichment in mutant ovary at 6 months. Similar follicle degeneration phenomenon was also observed in heterozygous mutant ovary at 8 months. The degenerating follicles in mutant were convinced by in situ apoptosis detection kit. To further explore molecular mechanism, RNA-seq and qPCR validation were adopted to identify underlying molecular pathway that involved in Cdkn1a deficient apoptosis. This study firstly reported the functions of Cdkn1a in zebrafish, particular in its critical role in maintenance of normal follicle development, and the special phenotypes in folliculogenesis perfectly interpretation of its dual roles on both proliferation and apoptosis at two different phases, which replenished its novel role in folliculogenesis that expanded our insights.

2024-10-27 | GSE239622 | GEO

Identification of a specific 13-miRNA expression signature during follicle activation in Zebrafish

Project description:Purpose: We aimed to investigate important miRNA events and to define specific miRNA expression signature underlying the follicle activation of zebrafish. Methods: By using small and regular RNA-sequencing, we performed transcriptomic analyses of PG (primary growth stage I; inactive) and PV (pre-vitellogenic stage II; activated) follicles to decipher important miRNA and gene events underlying follicle activation of zebrafish. We identified differentially expressed miRNAs for subsequent qPCR validation and miRNA::target gene prediction. Interaction of candidate miRNA:: target gene pairs were further validated by luciferase reporter assay. Global gene networks involved during PG-to-PV transition were also assessed by Gene Ontology as well as KEGG pathway analyses. Results and Conclusion: Our expression results indicated that PG follicles can be well differentiated from PV follicles by simply using a specific 13-miRNA expression signature (let-7a, -7b, -7c-5p, -7d-5p, -7h, -7i; miR-21, -23a, -27c-3p, -107a-3p, -125b-5p, -145-3p, -202-5p). Besides, we validated interactions of let-7i::atg4a, miR-202-5p::c23h20orf24 and miR-144::ybx1 by luciferase reporter assay. Purpose: we aimed to investigate important miRNA events and to define specific miRNA expression signature underlying the follicle activation of zebrafish

2017-12-20 | GSE92639 | GEO

Transcriptomic Analysis for Differentially Expressed Genes in Ovarian Follicle Activation and Puberty Onset in the Zebrafish

Project description:Puberty is a special transition period in sexual maturation, and it has been extensively studied in vertebrates in the past decades. In mammals, the initiation of puberty involves activation of numerous genes; however, there have been few comprehensive reports in small model teleosts such as the zebrafish. In the zebrafish, the onset of puberty in females is marked by the appearance of the first wave of pre-vitellogenic (PV) follicles in the ovary during sexual maturation. Using transcriptomics and real-time qPCR, this study was undertaken to investigate temporal gene expression differences between the primary growth (PG) follicles and pre-vitellogenic (PV) follicles, with particular emphasis on oocyte and follicular cell specific genes as well as several closely associated signaling pathways. Our results showed that totally 1082 genes were significantly upregulated and 530 evidently downregulated during the PG-PV transition, and among them were some well recognized biomarkers such as cyp19a1a, fshr, inha and inhbaa and some novel genes like notch3, amh, gadd45ga and lpl. Further gene ontology analysis showed that egg coat formation and steroid hormone mediated signaling pathway might be critical for follicle activation from PG to PV stage. In addition, KEGG identified several signaling pathways that might play pivotal roles in early folliculogenesis, including phosphatidylinositol signaling system, glycolsaminoglycan biosynthesis, RNA transport, and p53 signaling pathways. Overall, this study reported a comprehensive analysis for biomarker genes and potential pathways involved in PG-PV transition or follicle activation, which also marks female puberty onset in the zebrafish when occurring for the first time in sexual maturation.

2018-10-01 | GSE99308 | GEO

Drosophila YBX1 homolog YPS promotes ovarian germline stem cell development by preferentially recognizing 5-methylcytosine RNAs

Project description:5-Methylcytosine (m5C) is one of the most abundant RNA modifications, but its function in adult stem cell development remains poorly defined. This study shows that Ypsilon schachtel (YPS) promotes germ line stem cell (GSC) maintenance, proliferation, and differentiation in the adult Drosophila ovary by preferentially binding to m5C-containing RNAs. Highly conserved cold-shock domains (CSDs) of YPS and its human homolog Y box binding protein 1 (YBX1) exhibit preferential binding to m5C-containing RNAs through hydrophobic interactions. Human YBX1 can functionally replace YPS to promote GSC development in the Drosophila ovary, and overexpressing RNA-binding–defective YBX1 and YPS mutant proteins disrupts normal GSC development. Thus, this study provides insight into the importance of m5C RNA modification in adult stem cell development.

2020-02-03 | GSE139828 | GEO

Transcriptional profiling of somatic cells differentiation throughout oocyte competence acquisition in rainbow trout ovarian follicles.

2012-10-14 | GSE36602 | GEO

Transcriptional profiling of somatic cells differentiation throughout oocyte competence acquisition in rainbow trout ovarian follicles.

Project description:Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes). NC1 samples were collected from late vitellogenic females (LV), C1 samples from post-vitellogenic females (PV), and C2 samples from females undergoing meiotic maturation (Germinal Vesicle Breakdown) Global transcriptional profiling was performed using somatic cells collected from rainbow trout ovarian follicles during in vivo oocyte developmental competence acquisition. Somatic cells were collected at 3 stages of oogenesis: NC1 stage follicles (LV, late vitellogenic, prophase I arrested oocytes, meiotically incompetent and developmentally incompetent, n=6), C1 stage follicles (PV, post-vitellogenic, prophase I arrested oocytes, meiotically competent and developmentally competent, n=16). Ovulatory follicles were also collected during oocyte maturation after in vivo induction (metaphase II arrested oocytes, developmentally fully competent, n=6).

2012-10-14 | E-GEOD-36602 | biostudies-arrayexpress

Identification of chromatin-associated proteins occupying the promoter region of CDKN1A

Project description:The p21 protein, encoded by CDKN1A, plays a vital role in the induction of senescence, and its transcriptional control by p53 tumour supressor is well-established. However, p21 can also be regulated in a p53-independent manner, by mechanisms that remain poorly understood. Therefore, we here used a chromatin-directed proteomic approach and identified ZNF84 as a novel regulator of p21 in various p53-deficient cell lines.

2021-09-07 | PXD022542 | Pride

Comparative analysis of gene expression profiles between the ovary treated with or without Ci-TK

Project description:Tachykinins (TKs) and their receptors have been shown to be expressed in the mammalian ovary. However, the biological roles for ovarian TKs have yet to be verified. Ci-TK and Ci-TK-R, characterized from the protochordate (ascidian), Ciona intestinalis, are prototypes of vertebrate TKs and their receptors. In the present study, we show a novel biological function of TKs as an inducible factor for oocyte growth using C. intestinalis as a model animal. Immunostaining demonstrated the specific expression of Ci-TK-R in test cells residing in oocytes at the vitellogenic stage. DNA microarray and real-time PCR substantiated that Ci-TK induced gene expression of several proteases including cathepsin D, chymotrypsin, and carboxypeptidase B2 and functionally unidentified lectins or glycosidases in the ovary. The enzymatic activities of the above proteases in the ovary were also shown to be enhanced by Ci-TK. Of particular significance is that treatment of Ciona oocytes with Ci-TK resulted in progression of growth from the vitellogenic stage to the post-vitellogenic stage, which was completely blocked by a TK antagonist or protease inhibitors. These results led to the conclusion that Ci-TK enhances growth of the vitellogenic oocytes via up-regulation of gene expression and enzymatic activities of the proteases. Keywords: tachykinins, ovary

2008-08-19 | GSE8821 | GEO

Comparative analysis of gene expression profiles between the ovary treated with or without Ci-TK

2010-05-22 | E-GEOD-8821 | biostudies-arrayexpress