Effect of overexpressing E2FB in tomato plants
Ontology highlight
ABSTRACT:
ORGANISM(S): Solanum lycopersicum
PROVIDER: GSE23971 | GEO | 2010/09/04
SECONDARY ACCESSION(S): PRJNA130403
REPOSITORIES: GEO
Similar Datasets
Project description:The cell cycle transcription factor E2FB has been overexpressed in tomato plants (Solanum lycopersicum cv. Micro-Tom). This overexpression accelerates plant development and increases fruit yield. Total RNA was purified from true leaves and apical meristem of 14 days-old WT or E2FB-OE seedlings.
2010-09-03 | E-GEOD-23971 | biostudies-arrayexpress
Project description:Gene-to-gene coexpression analysis is a powerful approach to infer function of uncharacterized genes. To perform non-targeted coexpression analysis of tomato genes, we collected a developmental gene expression dataset using various tissues of tomato plant. Expression data are collected from 24 different tissue types including root, hypocotyl, cotyledon, leaf at different stages, and fruit tissues at 4 different ripening stages from 4 different Solanum lycopersicum cultivars. Fruits were separated to the flesh and the peel. These two tissue types indeed showed remarkably different gene expression profiles. We also collected data from 4 different ripening stages (mature green, yellow, orange, and red) to detail the changes during ripening. By using this gene expression dataset, we calculated pair-wise Pearsonâs correlation coefficients, and performed network-based coexpression analysis. The analysis generated a number of coexpression modules, some of which showed an enrichment of genes associated with specific functional categories. This result will be useful in inferring functions of uncharacterized tomato genes, and in prioritizing genes for further experimental analysis. We used Affymetrix GeneChip Tomato genome Arrays to detail the global gene expression change using 24 different tomato tissue types (67 hybridizations). We collected gene expression data from 24 different tomato tissue types using 67 hybridizations. Root, hypocotyl, cotyledon, and leaf were sampled from 3-week-old or 5-weekâold plant of Solanum lycopersicum cultivar Micro-Tom. Fruit tissues were sampled from S. lycopersicum cultivars Micro-Tom, Anthocyanin fruit (Aft, LA1996), Line27859, and Momotaro 8 (Takii, Japan). From Micro-Tom fruit, the peel and the flesh were separately sampled from 4 different ripening stages: mature green (MG, approximately 30 day after anthesis), yellow (Y, approximately 35 days after anthesis), orange (O, approximately 38-40 days after anthesis), and red (R, approximately 45-48 days after anthesis). From fruits of Aft and Line27859, the peel and the flesh were sampled at mature green (MG, approximately 40 days after anthesis) and red (R, approximately 50-55 days after anthesis) stages. From Momotaro 8, the peel and the flesh were sampled at red (R, 50- approximately 50-55 days after anthesis) stages. For each tissue type, 2-4 biological replicates were made in RNA preparation.
2010-02-21 | E-GEOD-19326 | biostudies-arrayexpress
Project description:Members of the tomato clade exhibit wide diversity in fruit coloration, growth habit, leaf morphology and mating preferences. However, the mechanisms governing inter-species diversity in fruit coloration are largely unknown. Therefore, a proteomic approach combined with carotenoid profiling and carotenogenic gene expression was used to decipher the diversity in carotenogenesis in green-fruited Solanum habrochaites, orange-fruited S. galapagense, and red-fruited S. pimpinellifolium with S. lycopersicum, cv. Ailsa Craig (tomato).
2017-10-09 | PXD003817 | Pride
Project description:The study was aimed to investigate by differential proteomics heat stress response mechanisms in tomato anthers collected from flowers of Solanum lycopersicum cv Saladette (SAL), already annotated as a thermo-tolerant tomato genotype and flowers from Solanum lycopersicum cv M82, reported as a thermo-sensitive genotype. Proteins constitutively present at higher or lower level in the tolerant genotype and/or proteins whose abundance was modulated by growth under high temperature in both genotypes were identified, thus leading to define processes involved in the heat stress response and responsible for efficient reduction of the adverse effects of HS. Results showed differences in the abundance of ninety-six proteins and their functional classification highlighted that heat stress mainly affected metabolic pathways, such as energy metabolism (glycolysis and sucrose degradation), nitrogen assimilation and amino acid biosynthesis and modulated the expression of proteins involved in the folding and degradation machinery and ROS detoxification systems
2018-07-25 | PXD010156 | Pride
Project description:Anthocyanins are specialized plant metabolites with significant dietary value due to their antioxidant and anti-inflammatory properties. Extensive research has indicated that dietary intake of these phenolic compounds contributes to preventing various chronic diseases. Consequently, incorporating anthocyanin-rich foods into one's diet, particularly from natural sources, is highly beneficial. The tomato (Solanum lycopersicum) is the most consumed vegetable worldwide, making it an excellent candidate for anthocyanin-enrichment strategies. The activation of anthocyanin biosynthesis is light-dependent in tomato, but this mechanism has not been entirely characterized. In this study, a purple tomato line in the cv. Micro-Tom (MT-Aft/atv/hp2) was utilized to investigate cyanic fruits developed under varying light conditions. This genotype is derived from natural genetic variation and exhibits anthocyanin accumulation starting early in fruit development. Transcriptional analyses of the fruit peel (exocarp or epicarp) and flesh (mesocarp) revealed that the bHLH transcription factor SlAN1 (Solyc09g065100) is the limiting factor for anthocyanin accumulation in both tissues. In this genotype, the absence of anthocyanin accumulation in the flesh results from the sun-blocking effect of the cyanic epicarp on the mesocarp, preventing light from penetrating deeper into the fruit during its development. This research enhances our comprehension of the genetic and environmental regulation of anthocyanin accumulation in fruit tissues, offering valuable insights for plant breeding and human nutrition.
2023-06-27 | GSE235565 | GEO
Project description:The two-spotted spider mite, Tetranychus urticae, is one of the most significant mite pests in agriculture that can feed on more than 1,100 plant hosts, including model plants Arabidopsis thaliana and tomato, Solanum lycopersicum. In order to refine the involvement of jasmonic acid (JA) in mite-induced responses, we analyzed transcriptional changes in tomato JA signaling mutant defenseless1 (def-1) upon JA treatment and spider mite herbivory. We used microarray to assess global gene expression in Solanum lycopersicum def-1 cv. Castlemart upon jasmonic acid treatment and Tetranychus urticae attack.
2015-01-05 | GSE61075 | GEO
Project description:RNA-seq of seedlings of four tomato species Solanum habrochaites, Solanum lycopersicum, Solanum pimpinelliolium, and Solanum pennellii. An additional panel of samples include many tissues from Solanum lycopersicum and Solanum pennellii in two light conditions
2013-04-05 | GSE45774 | GEO
Project description:The red spider mite, Tetranychus evansi, is a oligophagous specialist mite pest of Solanaceae plants. Here, we described tomato transcriptional responses to T. evansi feeding and compared them to responses to tomato-adapted and -non-adapted strains of generalist herbivorous spider mite Tetranychus urticae. We used microarray to assess global gene expression in Solanum lycopersicum cv. Heinz 1706 upon T. evansi attack.
2023-07-10 | GSE227740 | GEO
Project description:Tomato phototropin1 mutant exhibits deep red color in the ripe fruits compared to the wild type. However, the mechanisms governing this intense fruit coloration in the mutant are largely unknown. Therefore, a proteomic approach combined with carotenoid profiling and carotenogenic gene expression was used to decipher the carotenogenesis in a tomato phototropin1 mutant with S. lycopersicum, cv. Ailsa Craig (tomato).
2021-02-24 | PXD018150 | Pride
Project description:Generalist arthropod herbivores rapidly adapt to a broad range of host plants. However, the extent of transcriptional reprogramming in the herbivore and its hosts associated with adaptation remains poorly understood. Using the spider mite Tetranychus urticae and tomato as models with available genomic resources, we investigated the reciprocal genome-wide transcriptional changes in both spider mite and tomato as a consequence of mite’s adaptation to tomato We used microarray to assess global gene expression in Solanum lycopersicum cv. Moneymaker upon Tetranychus urticae attack by tomato-adapted and non-adapted spider mite lines.
2015-07-20 | GSE70375 | GEO