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The transcriptional regulator oxtR and its involvement in oxygen stress response in T. denticola

ABSTRACT: Treponema denticola has been strongly implicated in the pathogenesis of chronic periodontitis. Previously, we reported increased expression of TDE_0259 (oxtR), which exhibits similarities with the multiple-antibiotic resistance regulator (marR)-like gene in the bacteriocin ABC transporter gene-deficient mutant, which altered susceptibility to antimicrobial agents. Therefore, we investigated the function of oxtR in T. denticola using an oxtR-deficient mutant. We observed increased OxtR expression upon oxygen exposure. The growth rate of the bacterium was unaffected by the inactivation of oxtR. We observed a relative increase in the expression of genes associated with iron-sulfur cluster-binding proteins, flavodoxin, oligopeptide/dipeptide ABC transporters, heat shock proteins, DNA helicase, iron compounds, ABC transporters, and lipoproteins. The oxtR mutant exhibited an increased minimum inhibitory concentration against ofloxacin. In addition, the mutant showed a slightly faster growth rate than that of the wild type under oxygen stress. Our findings also suggested that OxtR may regulate the operon by binding to a potential promoter region. The oxygen-sensing regulator oxtR plays a role in regulating the expression of a potential ferredoxin, which may contribute to the response to oxygen-induced stress in T. denticola.

ORGANISM(S): Treponema denticola Treponema denticola ATCC 35405

PROVIDER: GSE240817 | GEO | 2024/08/07

REPOSITORIES: GEO

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