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Effect of Tsc2 depletion on gene expression in isolated mouse lung cells.

ABSTRACT: To evaluate the impact of mTOR activation by Tsc2KD in MVPC on the microvascular endothelial cell population we utilized single cell transcriptomic analysis of fractionated lung tissue. Mouse lung samples were pooled and cell sorting was 2 days and 10 weeks post tamoxifen induction. Following annotation of the original 28 lung subclusters, capillary MVEC were localized to 7 subclusters. Temporal comparison of the MVEC populations demonstrated that in WT the population was transcriptionally stable from 2 days to 10 weeks postinduction, as would be expected during angiostasis. In contrast lung MVEC from the mice with mTOR+ Tsc2KD MVPC demonstrated significant increases in gene expression at 10 weeks relative to day 2 post-induction. Increased gene expression was related to mesenchymal stem cell differentiation, microvascular endothelial differentiation, mTOR activation, autophagy and apoptosis. These detailed complementary analyses identify mechanistic consequences of Tsc2KD and mTOR dysregulation in MVPC.

ORGANISM(S): Mus musculus

PROVIDER: GSE242065 | GEO | 2023/10/31

REPOSITORIES: GEO

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Effect of Tsc2 depletion on gene expression in isolated mouse lung cells.

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