Kinetic chromatin openin profiles in mouse ApcDHep or wt hepatocytes
Ontology highlight
ABSTRACT: We analyzed chromatin opening in hepatocytes isolated from Apclox/loxlivers, after injection of adenovirus Ad5 -Cre-GFP and sorting on the basis of GFP signal at day 6, 15 and 21 post-injection. Our aims was to study the impact of an aberrant beta-catenin activation on chromatin opening in hepatocytes during the early steps of liver tumorigenesis.
Project description:We analyzed the expression of RNAs in hepatocytes isolated from Apclox/lox livers, after injection of adenovirus Ad5-Cre-GFP and hepatocyte sorting on the basis of GFP signal at day 6, 15 and 21 post-injection. Our aims was to study the transcriptional impact of the beta-catenin during the early steps of liver tumorigenesis.
Project description:We analyzed the level of DNA methylation by MeDIPseq in hepatocytes isolated by collagenase perfusion from Apclox/loxlivers, six days after injection of 2mg tamoxifen vs wt hepatocytes. Our aims was to study the impact of the beta-catenin on DNA methylation during the early steps of liver tumorigenesis
Project description:We analyzed chromatin opening in hepatocytes isolated from ApcDeletaHep livers or wt by ATAC-seq. We thus explore how an aberrant activation of beta-catenin signaling modifies chromatin structure in hepatocytes
Project description:Hepatocyte specific TBG promoter-driven Cre,GFP and YAP S127A expression was achieved by retro orbital injection of AAV. RNA-seq was performed for hepatocytes isolated by FACS.
Project description:Initiated hepatocytes (IHCs), isolated from DEN exposed Tgfbr2 flox/flox mouse, were infected with GFP or Cre-expressed adenovirus, followed by 1ng/ml TGFβ × 48 hours incubation. Then total RNAs were isolated and processed to routine sequencing on Illumina platform. RNA-Seq libraries were prepared from total RNA using polyA enrichment.
Project description:The effect of PGC-1α overexpression using adenovirus (PGC-1α-Ad) on hepatic gene expression was studied in mice primary hepatocytes.
Project description:A "Cartes d'Identite des Tumeurs" (CIT) project from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net). Conditional Knockout mouse model for the tumor suppressor gene Ap, Apc 2lox. The disruption of the Apc gene is realized by injection of an adenovirus encoding the Cre recombinase (AdCre). Homozygous animals injected with a high dose of AdCre showed an activation of the Wnt/beta-catenin signaling in most of the hepatocytes and develop an hepatomegaly and die soon after. Analysis of gene up- and down regulation for liver-indicible APC disruption.
Project description:To investigate the effect of TRIB3 overexpression on regulation of lipid metabolism in hepatocytes, we isolated mouse primary hepatocytes from AAV-GFP or AAV-Trib3 mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of two groups of mouse primary hepatocytes from AAV-GFP or AAV-Trib3 mice.
Project description:Transcriptome analysis of total blood cells from sheep collected at 0, 4 and 24 hours after vaccination with inactivated FMDV vaccine, and adenovirus (human Ad5)-based vaccines encoding FMDV antigens, with and without Montanide ISA206 VG adjuvant.