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Single-Cell Transcriptomic Profiling of Human iPSC-Derived Macrophage Differentiation Under Distinct Cytokine Conditions

ABSTRACT: The differentiation of human induced pluripotent stem cells (iPSCs) into macrophages is a complex process involving the emergence of diverse cell types and progenitors. In this study, we conducted a comprehensive single-cell transcriptomic analysis to elucidate the dynamics of iPSC-to-macrophage differentiation and explore the heterogeneity of arising cell populations. Two different cytokine conditions were employed to modulate differentiation, and we hypothesized that these conditions would yield distinct cell compartments. Our results reveal a nuanced cellular trajectory from iPSCs to macrophages, uncovering key intermediates and transitional states. Notably, the emerging cell populations remain consistent, albeit with variations in their proportions, with IL-3-only condition exhibiting a lower proportion of mature cells. This investigation advances our understanding of iPSC-to-macrophage differentiation, shedding light on the cellular heterogeneity and trajectory dynamics. The findings have implications for the development of targeted differentiation strategies and the exploration of novel therapeutic applications in regenerative medicine and immunology.

ORGANISM(S): Homo sapiens

PROVIDER: GSE242972 | GEO | 2024/02/15

REPOSITORIES: GEO

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