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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ex_vivo_ILC2]

ABSTRACT: The goal of this study was to determine whether thymic innate lymphoid cells type-2 (ILC2) activation during acute thymic involution is mediated by the alarmins IL-25 and IL-33. For this purpose we sorted thymic ILC2 from WT mice, treated them ex vivo with recombinant IL-25/IL-33 or both for 24 hours, and performed bulk RNA Seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE244222 | GEO | 2024/01/11

REPOSITORIES: GEO

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ILC2_after_Dex]

Project description:The goal of this study was to understand the role of thymic innate lymphoid cells (ILC2) in thymic regeneration following dexamethasone-induced acute involution, and determine whether this is activated by the alarmins IL-25 and IL-33. For this purpose we treated WT, Il25 KO or Il33 mice with dexamethasone, sorted ILC2 cells from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA-Seq.

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ex_vivo_tuft]

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Tuft_after_Dex]

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