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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [TEC_after_rAREG_inj]

ABSTRACT: The goal of this study was to determine how thymic epithelial cells (TEC) differentiation and gene expression is affected by amphiregulin (AREG), which is produced by ILC2 upon thymic involution and the receptor for which is expressed by early TEC subsets. For this purpose we treated WT mice with recombinant AREG, sorted TEC from their thymi 10 days after treatment when the thymus is recovering and AREG has been released, and performed bulk RNA-Seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE244225 | GEO | 2024/01/11

REPOSITORIES: GEO

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ILC2_after_irr]

Project description:The goal of this study was to understand the role of thymic innate lymphoid cells (ILC2) in thymic regeneration following irradiation-induced acute involution, and determine whether this is activated by the alarmin IL-25. For this purpose we treated WT or Il25 KO mice with sublethal irradiation and/or recombinant IL-25, sorted ILC2 cells from their thymi 3 days after treatment when the thymus begins its recovery, and performed bulk RNA Seq.

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Tuft_after_Dex]

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Tuft_after_irr]

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