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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [CITE-Seq]

ABSTRACT: The goal of this study was to examine the effect of dexamethasone-induced involution on the thymus cellular composition, and highlight cell populations which participate in the regeneration process. For this purpose we treated WT mice with dexamethasone, sorted non-T hematopoietic cells from their thymi 4 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed CITE-Seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE244229 | GEO | 2024/01/11

REPOSITORIES: GEO

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Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Fibroblasts_after_Dex]

Project description:The goal of this study was to understand the role of fibroblasts in thymic regeneration following dexamethasone-induced acute involution. For this purpose we treated WT mice with dexamethasone, sorted fibroblasts from their thymi 4 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA Seq.

2024-01-11 | GSE244218 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [NKT_after_Dex]

Project description:The goal of this study was to understand the role of thymic natural killer T cells (NKT) in thymic regeneration following dexamethasone-induced acute involution. For this purpose we treated WT mice with dexamethasone, sorted NKT from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA Seq.

2024-01-11 | GSE244221 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Tuft_after_Dex]

Project description:The goal of this study was to understand the role of thymic tuft cells in thymic regeneration following dexamethasone-induced acute involution, and determine whether this is affected by IL-13, which is produced by ILC2 upon involution and the receptor for which is expressed by tuft cells. For this purpose we treated WT and Il13ra1 KO mice with dexamethasone, sorted tuft cells from their thymi 3 days after treatment when the thymus begins its recovery, at an earlier time point (18 hours) for comparison, or at a later time point (6d) when IL-13 has been released, and performed bulk RNA Seq.

2024-01-11 | GSE244220 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ILC2_after_Dex]

Project description:The goal of this study was to understand the role of thymic innate lymphoid cells (ILC2) in thymic regeneration following dexamethasone-induced acute involution, and determine whether this is activated by the alarmins IL-25 and IL-33. For this purpose we treated WT, Il25 KO or Il33 mice with dexamethasone, sorted ILC2 cells from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (18 hours) for comparison, and performed bulk RNA-Seq.

2024-01-11 | GSE244216 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Fibroblasts_after_irr]

Project description:The goal of this study was to understand the role of fibroblasts in thymic regeneration following irradiation-induced acute involution. For this purpose we treated WT mice with sublethal irradiation, sorted fibroblasts from their thymi 3 days after treatment when the thymus begins its recovery, or at an earlier time point (1 day) for comparison, and performed bulk RNA Seq.

2024-01-11 | GSE244217 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [Tuft_after_irr]

Project description:The goal of this study was to understand the role of thymic tuft cells in thymic regeneration following irradiation-induced acute involution. For this purpose we treated WT mice with sublethal irradiation, sorted tuft cells from their thymi 3 days after treatment when the thymus begins its recovery, and performed bulk RNA Seq.

2024-01-11 | GSE244219 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [ILC2_after_irr]

Project description:The goal of this study was to understand the role of thymic innate lymphoid cells (ILC2) in thymic regeneration following irradiation-induced acute involution, and determine whether this is activated by the alarmin IL-25. For this purpose we treated WT or Il25 KO mice with sublethal irradiation and/or recombinant IL-25, sorted ILC2 cells from their thymi 3 days after treatment when the thymus begins its recovery, and performed bulk RNA Seq.

2024-01-11 | GSE244202 | GEO

Tuft cells and fibroblasts promote thymus regeneration through activation of a type-2 immune response [TEC_after_rAREG_inj]

Project description:The goal of this study was to determine how thymic epithelial cells (TEC) differentiation and gene expression is affected by amphiregulin (AREG), which is produced by ILC2 upon thymic involution and the receptor for which is expressed by early TEC subsets. For this purpose we treated WT mice with recombinant AREG, sorted TEC from their thymi 10 days after treatment when the thymus is recovering and AREG has been released, and performed bulk RNA-Seq.

2024-01-11 | GSE244225 | GEO

Human Early Postnatal Thymus [CITE-seq]

Project description:Human Early Postnatal Thymus [CITE-seq]

| PRJNA1103190 | ENA

Human Early Postnatal Thymus [CITE-seq]

Project description:Within the thymus, regulation of the cellular cross-talk directing T cell development is dependent on spatial interactions within specialized niches. To create a holistic, spatially defined map of tissue niches guiding postnatal T cell development we employed the multidimensional imaging platform CO-detection by indEXing (CODEX), as well as Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) and Assay for Transposable-Accessible Chromatin (ATAC-seq). We generated age-matched 4–5-month-old postnatal thymus datasets for both male and female donors, and describe signaling and cell-cell interaction networks in sequential thymocyte developmental niches. Together, these data represent a unique age-matched spatial multiomic resource to investigate how sex-based differences in thymus regulation and T cell development arise, and provide an essential resource to understand the mechanisms underlying immune function and dysfunction in males and females.

2024-08-30 | GSE264587 | GEO

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