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Regulatory T cell adoptive transfer alters uterine macrophage populations, increasing a novel MHC-II-low macrophage associated with healthy pregnancy.

ABSTRACT: To investigate local immune mechanisms of intrauterine fetal demise (IUFD), we used the CBA mouse strain, which naturally has mid-late gestation fetal loss. We performed a Treg adoptive transfer and interrogated both pregnancy outcomes and the impact of systemic maternal Tregs on mucosal immune populations at the maternal-fetal interface. Treg transfer prevented fetal loss and increased an MHC-IIlow population of uterine macrophages. Single-cell RNA-sequencing was utilized to precisely evaluate the impact of systemic Tregs on uterine myeloid populations. A population of C1q+, Trem2+, MHC-IIlow uterine macrophages were increased in Treg-recipient mice. The transcriptional signature of this novel uterine macrophage subtype is enriched in multiple studies of human healthy decidual macrophages, suggesting a conserved role for these macrophages in preventing fetal loss.

ORGANISM(S): Mus musculus

PROVIDER: GSE244616 | GEO | 2023/10/09

REPOSITORIES: GEO

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