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Establishing murine trophoblast organoids as a platform for CRISPR-Cas9 screening [sgRNA]

ABSTRACT: Murine trophoblast organoids contain trophoblast cell types. We used CRISPR SCREENING to analyze the trophoblast organoids derived from TS (mTOs).

ORGANISM(S): Mus musculus

PROVIDER: GSE245217 | GEO | 2023/10/25

REPOSITORIES: GEO

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Establishing murine trophoblast organoids as a platform for CRISPR-Cas9 screening [scRNA-seq]

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Establishing murine trophoblast organoids as a platform for CRISPR-Cas9 screening

Project description:This SuperSeries is composed of the SubSeries listed below.

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scRNA-seq and snucRNA-seq of human primary trophoblast organoids (PTO) and trophoblast stem cells (TSCs) differentiation into extravillous trophoblast organoids (EVTs)

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Project description:Trophoblast organoids derived from placental villi provide a 3D model system of human placental development, but access to first-trimester tissues is limited. Here we report that trophoblast stem cells isolated from naïve human pluripotent stem cells (hPSCs) can efficiently self-organize into 3D stem cell-derived trophoblast organoids (SC-TOs) with a villous architecture similar to primary trophoblast organoids. Single cell transcriptome analysis reveals the presence of distinct cytotrophoblast and syncytiotrophoblast clusters and a small cluster of extravillous trophoblasts, which closely correspond to trophoblast identities in the post-implantation embryo. These organoid cultures display clonal X chromosome inactivation patterns previously described in the human placenta. We further demonstrate that SC-TOs exhibit selective vulnerability to emerging pathogens (SARS-CoV-2 and Zika virus), which correlates with expression levels of their respective entry factors. The generation of trophoblast organoids from naïve hPSCs provides an accessible 3D model system of the developing placenta and its susceptibility to emerging pathogens.

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