Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect on gene expression of addition of N-acetylmannosamine to galactose grown Streptococcus pneumoniae.

ABSTRACT: RNA-seq analysis of N-acetylmannosamine upon growth and gene expression in pneumococci grown with galactose as sole sugar.

ORGANISM(S): Streptococcus pneumoniae

PROVIDER: GSE246424 | GEO | 2023/11/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional response of Streptococcus pneumoniae D39 to Galactose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 wild-type grown in M17 medium + 0.5 % (w/v) Galactose (GaM17) to grown in M17 medium + 0.5 % (w/v) Glucose (GM17).

2014-06-20 | GSE58180 | GEO

Transcriptome comparison of the Streptococcus pneumoniae D39 ∆rgg144to D39 wild type grown in galactose

Project description:Microbes communicate with each other by using quorum sensing (QS) systems and modulate their collective ‘behavior’ for in-host colonization and virulence, biofilm formation, and environmental adaptation. The recent increase in genome data availability reveals the presence of several putative QS sensing circuits in microbial pathogens, but many of these have not been functionally characterized yet, despite their possible utility as drug targets. To increase the repertoire of functionally characterized QS systems in bacteria, we studied Rgg144/Shp144 and Rgg939/Shp939, two putative QS systems in the important human pathogen Streptococcus pneumoniae. We find that both of these QS circuits are induced by short hydrophobic peptides (Shp) upon sensing sugars found in the respiratory tract, such as galactose and mannose. Microarray analyses using cultures grown on mannose and galactose revealed that the expression of a large number of genes is controlled by these QS systems, especially those encoding for essential physiological functions and virulence-related genes such as the capsular locus. Moreover, the array data revealed evidence for cross-talk between these systems. Finally, these Rgg systems play a key role in colonization and virulence, as deletion mutants of these QS systems are attenuated in the mouse models of colonization and pneumonia.

2018-10-19 | GSE112283 | GEO

Transcriptome comparison of the Streptococcus pneumoniae D39 ∆rgg939 to D39 wild type grown in galactose

2018-10-19 | GSE112285 | GEO

Comparing transcriptome by means of DNA microarrays of Streptococcus pneumoniae D39 grown with galactose to cells grown with glucose as carbon source

Project description:Streptococcus pneumoniae is a strictly fermentative human pathogen that relies on carbohydrate metabolism to generate energy for growth. The nasopharynx colonised by the bacterium is poor in free sugars, but mucosa lining glycans can provide a source of sugar. In blood and inflamed tissues glucose is the prevailing sugar. As a result during progression from colonisation to disease S. pneumoniae has to cope with a pronounced shift in carbohydrate nature and availability. Thus, we set out to assess the pneumococcal response to sugars found in glycans and the influence of glucose (Glc) on this response at the transcriptional, physiological and metabolic levels. Galactose (Gal), N-acetylglucosamine (GlcNAc) and mannose (Man) affected the expression of 8 to 14% of the genes covering cellular functions including central carbon metabolism and virulence. The pattern of end-products as monitored by in vivo 13C-NMR is in good agreement with the fermentation profiles during growth, while the pools of phosphorylated metabolites are consistent with the type of fermentation observed (homolactic vs. mixed) and regulation at the metabolic level. Furthermore, the accumulation of -Gal6P and Man6P indicate metabolic bottlenecks in the metabolism of Gal and Man, respectively. Glc added to cells actively metabolizing other sugar(s)was readily consumed and elicited a metabolic shift towards a homolactic profile. The transcriptional response to Glc was large (over 5% of the genome). In central carbon metabolism (most represented category), Glc exerted mostly negative regulation. The smallest response was observed on a sugar mix, suggesting that exposure to varied sugars improves the fitness of S. pneumoniae. The expression of classical virulence factors was negatively controlled by Glc in a sugar-dependent manner. Overall, our results shed new light on the link between carbohydrate metabolism, adaptation to host niches and virulence.

2015-09-01 | GSE70639 | GEO

Impact of galR delation on the transcriptome of Streptococcus pneumoniae D39 in the presence of Galactose

Project description:Transcriptome comparison of the Streptococcus pneumoniae D39 ΔgalR to D39 wild type grown in M17 medium+ 0.5% (w/v) galactose (GalM17).

2015-10-30 | GSE74501 | GEO

ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + Galactose at MID-log growth phase

Project description:Comparison of Streptococcus pneumoniae D39 ccpA mutant compared to D39 wild type in CDM with galactose as sole carbon source to define the regulon of carbon catabolite control protein CcpA under this condition Details described in Carvalho SM, Kloosterman TG, Neves AR, Kuipers OP. CcpA Ensures Optimal Metabolic Fitness of Streptococcus pneumoniae. Plos One 2011

2011-10-01 | GSE31817 | GEO

Neuraminidase-exposed galactose promotes Streptococcus pneumoniae biofilm formation during colonization

Project description:Galactose promotes pneumococcal biofilms in vivo 15 mRNA profiles of Streptococcus pneumoniae samples that were grown under different conditions were generated using deep sequencing.

2016-08-04 | E-GEOD-85196 | biostudies-arrayexpress

GalR Acts as a Transcriptional Activator of galKT in the Presence of Galactose in Streptococcus pneumoniae

Project description:We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of Δ galK with the D39 wildtype strain grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose. This SuperSeries is composed of the SubSeries listed below.

2015-10-30 | GSE74502 | GEO

Neuraminidase-exposed galactose promotes Streptococcus pneumoniae biofilm formation during colonization

Project description:Galactose promotes pneumococcal biofilms in vivo

2016-08-04 | GSE85196 | GEO

ccpA mutant compared to D39 wild-type in Streptococcus pneumoniae in CDM + galactose at transition-phase of growth (TS)

2011-10-01 | GSE31818 | GEO