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Effect of deleting ultra-conserved elements PAX6_Tarzan, PBX3_Claudia on gene expression in K562 cells

ABSTRACT: Non-coding regulatory elements (NCRE) represent a major fraction of the human genome, play important roles in different biological pathways, and have the potential as genomic medicine targets. We developed a straightforward dual-CRISPR screening system capable of deleting thousands of NCREs genome-wide to study their functions in distinct biological contexts in K562 cells. Here we reported 4 top hits from the genome-wide screen of ultra-conserved elements (UCE), i.e. PAX6_Tarzan(chr11:31810437-31810870, hg19) and PBX3_Claudia(chr9:128457564-128457785, hg19) play essential roles in cell growth and drug response.

ORGANISM(S): Homo sapiens

PROVIDER: GSE247234 | GEO | 2024/03/10

REPOSITORIES: GEO

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