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Brd4 knockout colonic epithelial organoids are partially resistant to IFNg-induced cell death.

ABSTRACT: To identify the role of Brd4 in colorectal cancer (CRC) development , we performed Sleeping Beauty (SB) transposon mutagenesis in mice. We identified several candidate genes including Brd4. To provide functional validation, we knocked out Brd4 in mouse tumor organoids carrying an activating KrasG12D, and an inactivating ApcD716 allele, and performed RNAseq. Brd4 knockout colonic organoids showed deregulation of genes in IFNg signaling. We treated Brd4 knockout organoids with IFNg and found that Brd4 konckout organoids showed partial resistance to IFNg-induce cell death. The mechanism may explain the function of Brd4 in colon cancer development.

ORGANISM(S): Mus musculus

PROVIDER: GSE248821 | GEO | 2024/11/24

REPOSITORIES: GEO

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