Genomics

Dataset Information

0

MiR-193b Regulates Mcl-1 in Melanoma


ABSTRACT: MicroRNAs (miRNAs) have important roles in gene regulation. Dysregulation of miRNAs has been associated with tumorigenesis. Recent studies suggest miR-193b is a tumor suppressor gene. In a previous study, we reported that miR-193b represses cell proliferation and regulates cyclin D1 (CCND1) in melanoma. Now we demonstrate that miR-193b regulates myeloid cell leukemia sequence 1 (Mcl-1) in melanoma cells. miRNA microarray profiling revealed the miR-193b level in malignant melanomas was significantly downregulated compared to benign nevi, while a tissue microarray demonstrated overexpression of Mcl-1 in malignant melanoma. The Mcl-1 expressions were inversely correlated with the miR-193b levels in melanoma tissue samples, suggesting a potential regulatory role of miR-193b. Overexpression of miR-193b repressed Mcl-1 in melanoma cell lines. It is well known that Mcl-1 knockdown confers cell sensitivity to ABT-737, a small molecular inhibitor of Bcl-2, Bcl-XL and Bcl-w. We found miR-193b, through repressing Mcl-1 expression, could also sensitize melanoma cells that were refractory to ABT-737. Furthermore, miR-193b directly regulates Mcl-1 by targeting the 3’ untranslated region (3’UTR) of Mcl-1 mRNA. Interestingly, miR-193b may recognize sequences on the 3’UTR that do not base pair with its seed region. In conclusion, our study suggests the downregulation of miR-193b could be an early event during melanoma progression, and demonstrates miR-193b directly regulates Mcl-1 by targeting both seed and seedless sequences of the 3’ UTR.

ORGANISM(S): Homo sapiens

PROVIDER: GSE24996 | GEO | 2010/10/30

SECONDARY ACCESSION(S): PRJNA131979

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-10-30 | E-GEOD-24996 | biostudies-arrayexpress
2013-04-03 | E-GEOD-36938 | biostudies-arrayexpress
2013-04-03 | GSE36938 | GEO
| PRJNA131979 | ENA
2008-06-19 | E-GEOD-10841 | biostudies-arrayexpress
2008-05-01 | GSE10841 | GEO
2008-12-19 | GSE10003 | GEO
2011-07-01 | E-GEOD-25613 | biostudies-arrayexpress
2010-05-16 | E-GEOD-18512 | biostudies-arrayexpress
2024-10-01 | GSE235574 | GEO