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Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

ABSTRACT: To investigate transcriptome changes of endothelial cells during cancer cell response, we studied HUVEC RNA expression in conditioned medium derived from empty plates versus B16-F10 melanoma cultures.

ORGANISM(S): Homo sapiens

PROVIDER: GSE251979 | GEO | 2024/09/11

REPOSITORIES: GEO

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Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

Project description:Endothelial cells (ECs) form a tissue-specific barrier for disseminating cancer cells in distant organs. However, the molecular regulation of the ECs in the metastatic niche is poorly understood. Here, we analyzed the transcriptional reprogramming of ECs in the lung metastatic niche six hours after the arrival of melanoma cells in the niche. We discovered a novel EC cluster derived from venous capillaries in response to infiltrating cancer cells, which was enriched for cell-cell communication pathways, including the Dll4, Vegf and Il6-Jak-Stat pathways. The Jak-Stat activated oncogenic Pim3 kinase was a marker of the cancer responding ECs, being upregulated in spontaneous metastasis models and in ECs of human cancer. Notably, PIM inhibition increased vascular leakage and metastatic colonization in vivo and impaired the EC barrier via decreased junctional Cadherin-5 and catenins a, b and d. Thus, PIM3 protects the EC barrier in the metastatic niche, which may impair the efficacy of PIM inhibitors in cancer therapies.

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Endothelial Pim3 kinase protects the vascular barrier during lung metastasis (set3)

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Endothelial Pim3 kinase protects the vascular barrier during lung metastasis (set2)

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proteome analysis of Lung tissue of mice bearing B16-F10-luc-G5 melanoma tumor with sleep fragmentation and with or with out the asdmistration of GL-pp

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Transcription profiling of mouse model of melanome secreting different amounts of histidine

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Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

Project description:Endothelial Pim3 kinase protects the vascular barrier during lung metastasis

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