Genomics

Dataset Information

0

DNA Polymerase Delta Governs Parental Histone Transfer to DNA Replication Lagging Strand


ABSTRACT: Chromatin replication is intricately intertwined with DNA replication, and the recycling of parental histones is essential for epigenetic inheritance. The transfer of parental histones to both the DNA replication leading and lagging strands involves two distinct pathways: the leading strand utilizes DNA polymerase ε subunits Dpb3/Dpb4, while the lagging strand is facilitated by the MCM helicase subunit Mcm2. However, the process by which Mcm2, moving along the leading strand, facilitates the transfer of parental histones to the lagging strand remains unclear. Our study reveals that the deletion of Pol32, a non-essential subunit of major lagging-strand DNA polymerase δ, results in a predominant transfer of parental histone H3-H4 to the replication leading strand. Biochemical analyses further demonstrate that Pol32 can bind histone H3-H4 both in vivo and in vitro. The interaction of Pol32 with parental histone H3-H4 is disrupted by the mutation of Mcm2's histone H3-H4 binding domain. In conclusion, our findings identify the DNA polymerase delta subunit Pol32 as a novel key histone chaperone downstream of Mcm2, mediating the transfer of parental histones to the lagging strand during DNA replication.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE252049 | GEO | 2024/04/24

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-09-24 | GSE118580 | GEO
2024-08-01 | GSE241062 | GEO
2024-08-01 | GSE241013 | GEO
2024-08-01 | GSE241063 | GEO
2024-05-31 | GSE203465 | GEO
2024-04-24 | GSE240331 | GEO
2024-01-26 | PXD043392 | Pride
2020-09-02 | GSE142996 | GEO
2023-08-30 | PXD030364 | Pride
2023-08-30 | PXD020326 | Pride