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H3T11 phosphorylation by CKII is required for heterochromatin formation in Neurospora

ABSTRACT: Heterochromatin is a key feature of eukaryotic genomes. In the filamentous fungus Neurospora crassa, the mechanism leading to heterochromatin initiation is unknown. Here, we show that casein kinase II (CKII) are required for heterochromatin formation at the well-defined 5-kb heterochromatin of the 5H-cat-3 region. Similarly, mutation of the histone H3 phosphorylation site T11 also impairs heterochromatin formation at the same locus. The catalytic subunit CKA co-localizes with phosphorylated H3T11 (H3pT11) within the 5H-cat-3 domain and the deletion of cka results in a significant decrease in H3T11 phosphorylation. Furthermore, the loss of kinase activity of CKII results in a significant reduction of H3pT11, H3K9me3, and DNA methylation levels, suggesting that CKII regulates heterochromatin formation by promoting H3T11 phosphorylation. Together, our results establish that histone H3 phosphorylation by CKII is a critical event required for heterochromatin formation.

ORGANISM(S): Neurospora crassa

PROVIDER: GSE252700 | GEO | 2024/09/18

REPOSITORIES: GEO

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