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Transcriptome-wide Identification of RNA Bound by IGF2BP3 in Mouse Testes by eCLIP-seq

ABSTRACT: The knockout of IGF2BP3 affects the proper progress of spermatogenesis. We use Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) to find the targets bound by IGF2BP3 in the testis. After conducting joint analysis with the RNC-seq of knockout mice, we identified that changes in the downstream target genes of IGF2BP3 at the transcriptional and translational levels will damage the conversion of histones to protamines.

ORGANISM(S): Mus musculus

PROVIDER: GSE256053 | GEO | 2025/02/05

REPOSITORIES: GEO

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