Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Screening of NFIL3 target genes (Affymetrix HTA 2.0 data set)

ABSTRACT: To identify the target gene of NFIL3, we transfected K562 cells with siNFIL3 or siControl, respectively. In order to better understand the function of NFIL3 gene, mRNA expression profiles were analyzed by using Affymetrix HTA2.0 Microarray in K562 cells transfected with siNFIL3 or siControl under hypoxia

ORGANISM(S): Homo sapiens

PROVIDER: GSE256294 | GEO | 2024/02/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Screening of NFIL3 target genes (Affymetrix HTA 2.0 data set)

Project description:Screening of NFIL3 target genes (Affymetrix HTA 2.0 data set)

| PRJNA1078882 | ENA

Differential requirement for Nfil3 during NK cell development

Project description:Nfil3 has multiple functions during conventional NK cell and thymic NK cell development. Nfil3 controls NK cell development from the earliest committed NK cell progenitor stage. Nfil3-/- prepro NK cells retained T-cell potential due to the failure to repress T cell specific genes. At later stages of NK cell development, Nfil3 regulates the formation of mature NK cells by promoting directly or indirectly the expression of transcription factor Eomes. We also demonstrate that Nfil3 is important for thymic NK cell development whereas Trail+ immature NK cells generated in the bone marrow of neonates and in the adult liver develop independent of Nfil3 function. We used microarrays to analyse the changes in gene expression due to the presence or absence of Nfil3, both in prepro NK cells and ALP cells (all lymphoid progenitors)

2013-10-08 | GSE45955 | GEO

Differential requirement for Nfil3 during NK cell development

Project description:Natural killer (NK) cells can be grouped into distinct subsets that are localized to different organs and exhibit different capacity to secrete cytokines and mediate cytotoxicity. Despite these hallmarks that reflect tissue-specific specialization in NK cells, little is known about the factors that control the development of these distinct subsets. The basic leucine zipper transcription factor nuclear factor interleukin 3 (Nfil3; E4bp4) is essential for bone marrow-derived NK cell development but it is not clear whether Nfil3 is equally important for all NK cell subsets nor how it induces NK lineage commitment. Here we show that Nfil3 is required for the formation of Eomesodermin (Eomes)-expressing NK cells, including conventional medullary and thymic NK cells, whereas TRAIL+ Eomes- NK cells develop independent of Nfil3. Loss of Nfil3 during the development of bone marrow-derived NK cells resulted in reduced expression of Eomes and, conversely, restoration of Eomes expression in Nfil3-/- progenitors rescued NK cell development and maturation. Collectively, these findings demonstrate that Nfil3 drives the formation of mature NK cell by inducing Eomes expression and reveal the differential requirements of NK cell subsets for Nfil3.

2014-05-07 | GSE54291 | GEO

Differential requirement for Nfil3 during NK cell development

2013-10-08 | E-GEOD-45955 | biostudies-arrayexpress

Differential requirement for Nfil3 during NK cell development

2014-05-07 | E-GEOD-54291 | biostudies-arrayexpress

HEK293 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from HEK293 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (six replicates) and NFIL3 shRNA (four replicates)

2012-09-02 | E-GEOD-31426 | biostudies-arrayexpress

BT549 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (two replicates) and NFIL3 shRNA (two replicates)

2012-09-02 | E-GEOD-31425 | biostudies-arrayexpress

Diminishment of NFIL3 by shRNA in HEK293 and BT549 cells

Project description:This SuperSeries is composed of the following subset Series: GSE31425: BT549 cells with control or NFIL3 shRNA treatment GSE31426: HEK293 cells with control or NFIL3 shRNA treatment Refer to individual Series

2012-09-02 | E-GEOD-31427 | biostudies-arrayexpress

HEK293 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from HEK293 cells that were infected with control or NFIL3 shRNA

2012-09-02 | GSE31426 | GEO

BT549 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA

2012-09-02 | GSE31425 | GEO