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BT549 cells with control or NFIL3 shRNA treatment

ABSTRACT: Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA

ORGANISM(S): Homo sapiens

PROVIDER: GSE31425 | GEO | 2012/09/02

SECONDARY ACCESSION(S): PRJNA154185

REPOSITORIES: GEO

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BT549 cells with control or NFIL3 shRNA treatment

Project description:Transcriptional profiling with samples from BT549 cells that were infected with control or NFIL3 shRNA Two condition experiment: control (scr) shRNA (two replicates) and NFIL3 shRNA (two replicates)

2012-09-02 | E-GEOD-31425 | biostudies-arrayexpress

Diminishment of NFIL3 by shRNA in HEK293 and BT549 cells

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Project description:BT549 cells with control or NFIL3 shRNA treatment

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Project description:Nfil3 has multiple functions during conventional NK cell and thymic NK cell development. Nfil3 controls NK cell development from the earliest committed NK cell progenitor stage. Nfil3-/- prepro NK cells retained T-cell potential due to the failure to repress T cell specific genes. At later stages of NK cell development, Nfil3 regulates the formation of mature NK cells by promoting directly or indirectly the expression of transcription factor Eomes. We also demonstrate that Nfil3 is important for thymic NK cell development whereas Trail+ immature NK cells generated in the bone marrow of neonates and in the adult liver develop independent of Nfil3 function. We used microarrays to analyse the changes in gene expression due to the presence or absence of Nfil3, both in prepro NK cells and ALP cells (all lymphoid progenitors) Samples were obtained on two experimentation dates. Replicate 1 was processed in April 2012, replicate 2 was processed in May 2012.

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