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SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis II

ABSTRACT: We present SIGNAL-seq (Split-pool Indexing siGNalling AnaLysis by sequencing): a multiplexed split-pool combinatorial barcoding method that simultaneously measures RNA and post-translational modifications (PTMs) in fixed single cells from 3D solid-tumour models. SIGNAL-seq PTM measurements are equivalent to mass cytometry and RNA gene detection is analogous to split-pool barcoding scRNA-seq. By measuring both mRNA ligand-receptor pairs and PTMs in single cells, SIGNAL-seq simultaneously reveals both inter- and intra-cellular signalling in tumour microenvironment organoids.

ORGANISM(S): Homo sapiens

PROVIDER: GSE256404 | GEO | 2024/02/24

REPOSITORIES: GEO

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SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis I

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SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

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