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SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis

ABSTRACT: We present SIGNAL-seq (Split-pool Indexing siGNalling AnaLysis by sequencing): a multiplexed split-pool combinatorial barcoding method that simultaneously measures RNA and post-translational modifications (PTMs) in fixed single cells from 3D models. SIGNAL-seq PTM measurements are equivalent to mass cytometry and RNA gene detection is analogous to split-pool barcoding scRNA-seq. By measuring both mRNA ligand-receptor pairs and PTMs in single cells, SIGNAL-seq can simultaneously un- cover inter- and intra-cellular regulation of tumour microenvironment plasticity. This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE256405 | GEO | 2024/02/24

REPOSITORIES: GEO

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SIGNAL-seq: Multimodal Single-cell Inter- and Intra-cellular Signalling Analysis II

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