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Profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE25685 | GEO | 2010/11/30

SECONDARY ACCESSION(S): PRJNA135833

REPOSITORIES: GEO

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Profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

Project description:This SuperSeries is composed of the following subset Series: GSE25068: PcG/TrxG profiling of differentially aged adipose-derived mesenchymal stem cells GSE25069: Whole-genome microarray of long-term cultured adipose derived mesenchymal stem cells from differentially-aged mice GSE25679: microRNA profiling of mesenchymal stem cells from adipose tissue of differentially aged mice Refer to individual Series

2010-11-30 | E-GEOD-25685 | biostudies-arrayexpress

microRNA profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

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microRNA profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

Project description:The data presented here is part of a study aimed to investigate whether the gene expression profile of Polycomb, Trithorax and interacting molecules might be related to different states of differentiative potential. microRNA profiling (Exiqon miRCURY LNA array Version 9.2) has been performed on mesenchymal stem cells isolated from the inguinal fat pad of mice of 1 (n=2), 3(n=2), 6(n=2), 12(n=2) and 24(n=1) months of age.

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Profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

Project description:Profiling of mesenchymal stem cells from adipose tissue of differentially aged mice

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PcG/TrxG profiling of differentially aged adipose-derived mesenchymal stem cells

Project description:The microarray reported here is part of a study aimed to investigate whether the gene expression profile of Polycomb, Trithorax and interacting molecules (collectively referred to PcG/TrxG genes) might be related to different states of differentiative potential. In particular , this microarray profiles the expression of PcG/TrxG genes in adipose-derived mesenchymal stem cells isolated from the inguinal fat depot of mice of different age.

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Adipose-Derived Mesenchymal Stem Cells Restore Impaired Mucosal Immune Responses in Aged Mice.

Project description:It has been shown that adipose-derived mesenchymal stem cells (AMSCs) can differentiate into adipocytes, chondrocytes and osteoblasts. Several clinical trials have shown the ability of AMSCs to regenerate these differentiated cell types. Age-associated dysregulation of the gastrointestinal (GI) immune system has been well documented. Our previous studies showed that impaired mucosal immunity in the GI tract occurs earlier during agingthan is seen in the systemic compartment. In this study, we examined the potential of AMSCs to restore the GI mucosal immune system in aged mice. Aged (>18 mo old) mice were adoptively transferred with AMSCs. Two weeks later, mice were orally immunized with ovalbumin (OVA) plus cholera toxin (CT) three times at weekly intervals. Seven days after the final immunization, when fecal extract samples and plasma were subjected to OVA- and CT-B-specific ELISA, elevated levels of mucosal secretory IgA (SIgA) and plasma IgG antibody (Ab) responses were noted in aged mouse recipients. Similar results were also seen aged mice which received AMSCs at one year of age. When cytokine production was examined, OVA-stimulated Peyer's patch CD4+ T cells produced increased levels of IL-4. Further, CD4+ T cells from the lamina propria revealed elevated levels of IL-4 and IFN-? production. In contrast, aged mice without AMSC transfer showed essentially no OVA- or CT-B-specific mucosal SIgA or plasma IgG Ab or cytokine responses. Of importance, fecal extracts from AMSC transferred aged mice showed neutralization activity to CT intoxication. These results suggest that AMSCs can restore impaired mucosal immunity in the GI tract of aged mice.

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Whole-genome microarray of long-term cultured adipose derived mesenchymal stem cells from differentially-aged mice

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