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Cellular dynamics of pig-to-human kdiney xenotransplantation [single cell RNA-seq]

ABSTRACT: We performed comprehensive single-cell RNA sequencing (scRNA-seq) analyses of the two porcine xenografts and their contralateral untransplanted kidneys to dissect xenotransplantation-associated cellular dynamics and xenograft-recipient interactions. We additionally performed longitudinal bulk RNA-seq on core biopsies to dissect the time-resolved physiological changes of the xenografts and longitudinal scRNA-seq of peripheral blood mononuclear cells (PBMCs) to detect recipient’s immune responses across time.

ORGANISM(S): Sus scrofa Homo sapiens

PROVIDER: GSE257542 | GEO | 2024/06/30

REPOSITORIES: GEO

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Cellular dynamics of pig-to-human kdiney xenotransplantation [RNA-seq]

Project description:We performed comprehensive single-cell RNA sequencing (scRNA-seq) analyses of the two porcine xenografts and their contralateral untransplanted kidneys to dissect xenotransplantation-associated cellular dynamics and xenograft-recipient interactions. We additionally performed longitudinal bulk RNA-seq on core biopsies to dissect the time-resolved physiological changes of the xenografts and longitudinal scRNA-seq of peripheral blood mononuclear cells (PBMCs) to detect recipient’s immune responses across time.

2024-06-30 | GSE257541 | GEO

scRNA-seq of Gene-edited Porcine Kidneys Prior to Xenotransplantation into Cynomolgus Macaques

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Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [scRNA-Seq]

Project description:Progress in clinical translation of gene edited porcine kidney xenotransplantation is accelerating. However, kidney function entails more than removal of metabolic waste products and it is unknown if all endocrine functions will be faithfully reproduced by porcine kidneys in primate recipients. Seventeen cynomolgus macaques with survival >60days after life sustaining kidney xenotransplantation (LSKX) from gene edited Yucatan minipigs underwent measurement of xenograft growth and two kidney dependent endocrine pathways: renin-angiotensin-aldosterone-system (RAAS) and calcium-vitamin D-parathyroid-hormone. Clinical chemistries, plasma-renin-activity, Beta-C-terminal-telopeptide (CTx) assay, RNA-seq, single-cell RNA-seq, and serial ultrasonography were conducted and generalized linear models assessed statistical relationships. Xenografts transplanted from minipigs demonstrated only modest growth (estimated ~8.0% per year) and didn’t significantly contribute to recipient RAAS pathway. However, PTH-independent hypercalcemia and hypophosphatemia were observed and might be xenograft intrinsic suggesting close monitoring and timely intervention during the first month post-transplant. Further study of these phenotypes is warranted in designing prospective clinical trials.

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Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [RNA-Seq]

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scRNA-seq of Gene-edited Porcine Kidneys Prior to Xenotransplantation into Cynomolgus Macaques

Project description:scRNA-seq of Gene-edited Porcine Kidneys Prior to Xenotransplantation into Cynomolgus Macaques

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Cellular dynamics of pig-to-human kdiney xenotransplantation [RNA-seq]

Project description:Cellular dynamics of pig-to-human kdiney xenotransplantation [RNA-seq]

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Clinical and molecular correlation defines activity of physiological pathways in life-sustaining kidney xenotransplantation [scRNA-Seq]

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Serial xenotransplantation in NSG mice promotes a hybrid epithelial/mesenchymal gene expression signature and stemness in rhabdomyosarcoma cells

Project description:NSTS-11 cell line derived from a primary embryonal rhabdomyosarcoma was used for serial xenotransplantation in NSG mice in vivo. Subsequently, three cell lines, LTB1, LTB5, and LTB24, were derived from primary, secondary, and tertiary xenograft tumors, respectively. Gene expression profiling of all four cell lines was performed to identify changes in gene expression over the course of serial xenotransplantation, which was associated with a gradual increase of stemness of embryonal rhabdomyosarcoma cells. For each of the xenograft-derived cell lines, the parental NSTS-11 cell line served as a reference control.

2019-04-30 | E-MTAB-7664 | biostudies-arrayexpress

Cellular dynamics of pig-to-human kdiney xenotransplantation [single cell RNA-seq]

Project description:Cellular dynamics of pig-to-human kdiney xenotransplantation [single cell RNA-seq]

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Identification of novel genes involved in postnatal longitudinal muscle growth

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