Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global microRNA expression profiling of microdissected tissues from diseased and non-diseased pancreatic tissues

ABSTRACT: Global microRNA expression profiling of microdissected pancreatic tissues were collected using Agilent miRNA microarrays (G4470B, Sanger 10.1) carrying 723 individual human miRNA probes. Four different sources of RNA were analyzed: microdissected normal pancreatic ductal cells (ND, n=4),microdissected acinar cells (AC, n=4), macrodissected chronic pancreatitis (CP, n=5) and micordissected xenograft tissues derived from primary pancreatic ductal adenocarcinomas (PDAC, n=5).

ORGANISM(S): Homo sapiens

PROVIDER: GSE25820 | GEO | 2011/09/28

SECONDARY ACCESSION(S): PRJNA135631

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Global microRNA expression profiling of microdissected tissues from diseased and non-diseased pancreatic tissues

Project description:Global microRNA expression profiling of microdissected pancreatic tissues were collected using Agilent miRNA microarrays (G4470B, Sanger 10.1) carrying 723 individual human miRNA probes. Four different sources of RNA were analyzed: microdissected normal pancreatic ductal cells (ND, n=4),microdissected acinar cells (AC, n=4), macrodissected chronic pancreatitis (CP, n=5) and micordissected xenograft tissues derived from primary pancreatic ductal adenocarcinomas (PDAC, n=5). Four condition (AZ, ND, PDAC, CP), each condition is represented by 4 to 5 biological replicates

2011-09-28 | E-GEOD-25820 | biostudies-arrayexpress

Gene expression in pancreatic ductal adenocarcinoma

Project description:To further develop our understanding of the gene expression signature of pancreatic ductal adenocarcinoma Gene expression signatures in macrodissected resected pancreatic ductal adenocarcinoma specimens

2014-03-14 | E-GEOD-55643 | biostudies-arrayexpress

Transcription profiling of microdissected human microdissected pancreas ductal adenocarcinoma vs. microdissected normal duct cells

Project description:Comparison of the gene expression of microdissected pancreas ductal adenocarcinoma to microdissected normal duct cells

2006-12-24 | E-MEXP-950 | biostudies-arrayexpress

Gene expression from laser capture microdissected pancreatic cancer epithelium and stroma

Project description:This study used laser capture microdissection to obtain paired tumor epithelium and stroma RNA samples from human pancreatic ductal adenocarcinoma (PDA) frozen sections. Libraries were prepared using the Nugen Ovation RNA-Seq System V2 and sequenced to a depth of 30 million 100bp single-end reads. These data were used to model compartment-specific gene expression density on a genome-wide scale and build an algorithm for transcriptional devonvolution (ADVOCATE). RNA sequencing of macrodissected bulk PDA sections was performed on 15 samples in order to systematically compare TruSeq and NuGEN RNA-Seq libraries and (ii) correlate histopathological and molecular assessment of tumor composition.

2018-11-27 | GSE93326 | GEO

Transcriptomic Analysis of Laser Capture Microdissected Tumors of Pancreatic Ductal Adenocarcinoma

Project description:Transcriptomic Analysis of Laser Capture Microdissected Tumors of Pancreatic Ductal Adenocarcinoma

| PRJNA691527 | ENA

Goserelin and bicalutamide treatments alter the expression of microRNAs in prostate

Project description:30 patients were randomized equally into three groups: no treatment, bicalutamide (antiandrogen) 150 mg daily, and goserelin (GnRH agonist) 3.6 mg every 4 weeks. Following the neoadjuvant treatment for 12 weeks, patients underwent radical prostatectomy. Freshly frozen specimens were collected for expression profiling by human Agilent miRNA V2 microarray chips. Each array contained probe sets for 723 human miRNAs according to Sanger miRBase v 10.1

2012-06-01 | E-MEXP-3530 | biostudies-arrayexpress

Whole Exome Sequencing of Microdissected Pancreatic Cancer

Project description:Whole Exome Sequencing of Microdissected Pancreatic Cancer

| PRJNA289549 | ENA

Transcription profiling of microdissected human pancreatic tissues and pancreas cell lines (data in addition to E-MEXP-950)

Project description:Gene expression of pancreas ductal adenocarcinoma, normal duct cells, tumor stroma and stroma of chronic pancreatitis as well as of several cell lines. All primary tissue samples were microdissected. It is an addition of the data set E-MEXP-950.

2007-06-18 | E-MEXP-1121 | biostudies-arrayexpress

Immunohistochemical markers distinguishing cholangiocellular carcinoma from pancreatic ductal adenocarcinoma discovered by proteomic analysis of microdissected cells

Project description:Cholangiocellular carcinoma (CCC) and pancreatic ductal adenocarcinoma (PDAC) are two highly aggressive cancer types which arise from epithelial cells of the pancreatobiliary system. Due to their histologic and morphologic similarity, differential diagnosis between CCC and metastasis of PDAC located in the liver frequently proves an unsolvable issue for pathologists. Biomarkers with high specificity and sensitivity for the differentiation of these tumour types would therefore be a valuable tool, but so far none are available. Here, we address this problem by comparing microdissected CCC and PDAC tumour cells in a label-free proteomics approach. The novel biomarker candidates were subsequently verified by immunohistochemical staining of primary and secondary tumour tissue.

2015-12-10 | PXD001833 | Pride

Primary_pancreatic_ductal_adenocarcinoma_cases - samples

Project description:Cell line derived from microdissected primary pancreatic ductal adenocarcinoma tissues

| EGAD00010000051 | EGA