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The impact of inhibiting Dgat2 and Pnpla2 functional enhancers on the transcriptome of 3T3-L1 adipocytes.

ABSTRACT: In order to explore the impact of enhancer inhibition on adipocyte differentiation and triglyceride deposition at the transcriptome level, we infected cells with lentiviral vectors containing dCas9-KRAB-sgRNAs (experimental group) or dCas9-KRAB-Control (control group) at a multiplicity of infection (MOI) of 100 for 12 hours in 1 mL culture medium. Transcriptome analysis via RNA sequencing (RNA-seq) was conducted on dCas9-KRAB-Dgat2-En4, dCas9-KRAB-Pnpla2-En5, and dCas9-KRAB-Control cells at day 7 of differentiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE259270 | GEO | 2025/02/05

REPOSITORIES: GEO

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