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Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE259372 | GEO | 2024/12/26

REPOSITORIES: GEO

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Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation (smallRNAseq_human_AGO2_IP.8614)

Project description:We report the results of RNA-Seq from a pair of HCA-7 derived cells with low, CC, and high, CC-CR, cells as well as RNA-seq from AGO2 RNA immunoprecipitation from the two cell lines in order to identify potential targets of miR-100 and miR-125b. Results were used to identify potential mRNAs that were upregulated in CC cells (low miR-100 and miR-125b), compared to CC-CR cells. In addition, we searched for potential mRNA targets that were enriched in the AGO2 immunoprecipitation of CC-CR cells.

2024-12-26 | GSE259371 | GEO

2024-12-26 | GSE259370 | GEO

Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs.

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-12-26 | GSE259375 | GEO

Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs. (smallRNAseq_human_miR_KO.8556)

Project description:We report the results of RNA-Seq from an HCA-7 derived cell line with high levels of miR-100 and miR-125b (CC-CR) and knockout cell lines generated from the high expressing cells. CC-CR cells had miR-100, miR-125b or both miRNAs knocked out using CRISPR Cas9. Since miRNAs function to negatively regulate their target, the knockout cell lines should have the potential mRNA targets upregulated in comparison to CC-CR cells. Results were used to identify potential mRNAs that were upregulated in the knockout cell lines compared to CC-CR cells

2024-12-26 | GSE259374 | GEO

Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs (RNAseq_human_miR_KO.7223)

2024-12-26 | GSE259373 | GEO

Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation

Project description:Analysis of a pair of HCA-7 derived cells with low expressing (CC) or high expressing (CC-CR) levels of miR-100 and miR-125b and RNA collected from Ago2 RNA immunoprecipitation

| PRJNA1081376 | ENA

| PRJNA1081379 | ENA

| PRJNA1081378 | ENA

Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs.

Project description:Analysis of an HCA-7 derived cell line (CC-CR) and cell lines generated with knockout of miR-100, miR-125b, or both miRNAs.

| PRJNA1081375 | ENA

Overcoming melanoma resistance to vemurafenib by targeting CCL2-induced miR-34a, miR-100 and miR-125b.

Project description:In melanoma, the adaptative cell response to BRAF inhibitors includes altered patterns of cytokine production contributing to tumor progression and drug resistance. Among the factors produced by PLX4032-resistant melanoma cell lines, CCL2 was higher compared to the sensitive parental cell lines and increased upon drug treatment. CCL2 acted as an autocrine growth factor for melanoma cells, stimulating the proliferation and resistance to apoptosis. In patients, CCL2 is detected in melanoma cells in tumors and in plasma at levels that correlate with tumor burden and lactate dehydrogenase. Vemurafenib treatment increased the CCL2 levels in plasma, whereas the long-term clinical response was associated with low CCL2 levels.Increased CCL2 production was associated with miRNA deregulation in the resistant cells. miR-34a, miR-100 and miR-125b showed high expression in both resistant cells and in tumor biopsies that were obtained from treated patients, and they were involved in the control of cell proliferation and apoptosis. Inhibition of CCL2 and of the selected miRNAs restored both the cell apoptosis and the drug efficacy in resistant melanoma cells. Therefore, CCL2 and miRNAs are potential prognostic factors and attractive targets for counteracting treatment resistance in metastatic melanoma.

| S-EPMC4826216 | biostudies-literature

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