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Anaplasma phagocytophilum infected NB4 cells

ABSTRACT: THREE INDEPENDENT REPLICATES AND ARE THE CONTROL NON-INFECTED CELLS: GSM49939, GSM49940, GSM49941 THREE INFECTED INDEPEDENDENT REPLICATES: GSM49942, GSM49943, GSM49944 Keywords: ordered

ORGANISM(S): Homo sapiens

PROVIDER: GSE2600 | GEO | 2005/07/29

SECONDARY ACCESSION(S): PRJNA92015

REPOSITORIES: GEO

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Transcription profiling of human the treatments A. phagocytophilum-infected vs uninfected NB4 cells to determine the global impact of A. phagocytophilum infection in NB4 cells

Project description:THREE INDEPENDENT REPLICATES ARE THE CONTROL NON-INFECTED CELLS: GSM49939, GSM49940, GSM49941; THREE INFECTED INDEPEDENDENT REPLICATES: GSM49942, GSM49943, GSM49944

2007-09-08 | E-GEOD-2600 | biostudies-arrayexpress

Differential expression of inflammatory and immune response genes in sheep infected with Anaplasma phagocytophilum.

Project description:Anaplasma phagocytophilum is the causative agent of tick-borne fever (TBF) in ruminants and human, equine and canine granulocytic anaplasmosis. A. phagocytophilum modifies host gene expression and immune response. The objective of this work was to analyze differential gene expression in A. phagocytophilum-infected sheep using microarray hybridization and real-time RT-PCR in experimentally and naturally infected animals. Keywords: disease state analysis

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Promyelocytic cells response to Anaplasma phagocytophilum infection

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2005-02-25 | GSE1694 | GEO

Anaplasma phagocytophilum-infected ticks, Japan.

Project description:We report Anaplasma phagocytophilum infection of Ixodes persulcatus and I. ovatus ticks in Japan. Unique p44/msp2 paralogs (and/or 16S rRNA genes) were detected in tick tissues, salivary glands, and spleens of experimentally infected mice. These findings indicate the public health threat of anaplasmosis in Japan.

| S-EPMC3367361 | biostudies-literature

Gene expression profiles of European wild boar naturally infected with Anaplasma phagocytophilum.

2009-05-12 | GSE15766 | GEO

Global gene transcription analysis of Anaplasma phagocytophilum in tick and human cells using tiling microarrays

Project description:A whole genome tiling microarray based on the published sequence of the Anaplasma phagocytophilum (Ap) strain Hz genome was used to measure transcriptional activity of Ap grown to late stage in three cell lines representing the diversity of host cells naturally infected by Ap: two human cell lines, HL-60 (promyelocytic leukocyte) and HMEC-1 (microvascular endothelial), and the tick (Ixodes scapularis) cell line ISE6. Keywords: cell type comparison

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Global DNA methylation changes and differential gene expression in Anaplasma phagocytophilum-infected human neutrophils

Project description:Background: Anaplasma phagocytophilum is an obligate intracellular prokaryotic pathogen that both infects and replicates within human neutrophils. The bacterium represses multiple antimicrobial functions while simultaneously increasing proinflammatory functions by reprogramming the neutrophil genome. Previous reports show that many observed phenotypic changes are in part explained by altered gene transcription. We recently identified that large chromosomal regions of the neutrophil genome are differentially expressed during A. phagocytophilum infection. Because of this, we sought to determine whether gene expression programs altered by infection were the result of changes in the host neutrophil DNA methylome. Results: Within 24 h of infection, marked increases in DNA methylation were observed genome-wide as compared with mock-infected controls and pharmacologic inhibition of DNA methyltransferases resulted in decreased bacterial growth. New regions of DNA methylation were enriched at intron and exon junctions; however, intragenic methylation did not correlate with altered gene expression. In contrast, intergenic DNA methylation was associated with A. phagocytophilum-induced gene expression changes. Within the major histocompatibility complex locus on chromosome 6, a region with marked changes in infection-induced differential gene expression, new regions of methylation were localized to boundaries of active and inactive chromatin. Conclusions: These data strongly suggest that A. phagocytophilum infection, in addition to altering histone structure, alters DNA methylation and the epigenome of its host cell to promote survival and replication, providing evidence that such bacterial infection can radically alter the epigenome of its host cell.

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Gene expression profiles of European wild boar naturally infected with Anaplasma phagocytophilum.

Project description:Anaplasma phagocytophilum infects a wide variety of host species and causes the diseases granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever in ruminants. The objective of this research was to characterize differential gene expression in wild boar naturally infected with A. phagocytophilum by microarray hybridization using the GeneChipÂ® Porcine Genome Array Differential gene expression in wild boar naturally infected with A. phagocytophilum was chacarterized by microarray hybridization using the GeneChipÂ® Porcine Genome Array and real-time RT-PCR.

2009-05-12 | E-GEOD-15766 | biostudies-arrayexpress

Intrauterine Transmission of Anaplasma phagocytophilum in Persistently Infected Lambs.

Project description:Anaplasma phagocytophilum, which causes the disease tick-borne fever (TBF), is the most important tick-borne pathogen in European animals. TBF may contribute to severe welfare challenges and economic losses in the Norwegian sheep industry. The bacterium causes a persistent infection in sheep and several other animal species. The objective of this study was to investigate whether intrauterine transmission occurs in persistently infected sheep. The study included thirteen 5-6-month-old unmated ewes, of which twelve were experimentally infected with A. phagocytophilum (GenBank acc. no. M73220). Four to six weeks later, all ewes were mated, and nine became pregnant. Blood samples were collected from these ewes and their offspring. If the lamb died, tissue samples were collected. The samples were analyzed with real-time PCR (qPCR) targeting the msp2 gene. PCR-positive samples were further analyzed by semi-nested PCR and 16S rDNA sequencing. A total of 20 lambs were born, of which six died within two days. Six newborn lambs (30%) were PCR-positive (qPCR), of which one was verified by 16S rDNA sequencing. The present study indicates that intrauterine transmission of A. phagocytophilum in persistently infected sheep may occur. The importance of these findings for the epidemiology of A. phagocytophilum needs to be further investigated.

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Unidirectional suppression of Anaplasma phagocytophilum genotypes in infected lambs.

Project description:Five-month-old lambs were simultaneously infected with different doses of two 16S rRNA genetic variants of Anaplasma phagocytophilum and thereafter followed for clinical observation and blood sampling. The result of the study indicates a unidirectional suppression of genotypes in infected lambs, at least during a certain period of an A. phagocytophilum infection.

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