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Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis

ABSTRACT: Genomic imprinting, an epigenetic phenomenon leading to parent-of-origin-specific gene expression, has independently e v olv ed in the en- dosperm of flo w ering plants and the placenta of mammals—tissues crucial f or nurturing embry os. While transposable elements (TEs) frequently colocalize with imprinted genes and are implicated in imprinting establishment, direct in v estigations of the impact of de no v o TE transposition on genomic imprinting remain scarce. In this study, we explored the effects of chemically induced transposition of the Copia element ONSEN on genomic imprinting in Arabidopsis thaliana . Through the combination of chemical TE mobilization and doubled haploid induction, we gener- ated a line with 40 new ONSEN copies. Our findings reveal a preferential targeting of maternally expressed genes (MEGs) for transposition, aligning with the colocalization of H2A.Z and H3K27me3 in MEGs—both previously identified as promoters of ONSEN insertions. Additionally, we demonstrate that chemically-induced DNA hypomethylation induces global transcriptional deregulation in the endosperm, leading to the breakdo wn of MEG imprinting. T his study pro vides insights into the consequences of chemically induced TE remobilization in the endosperm, re v ealing that chemically-induced epigenome changes can ha v e long-term consequences on imprinted gene expression.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE260819 | GEO | 2024/06/18

REPOSITORIES: GEO

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Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis

Project description:Genomic imprinting, an epigenetic phenomenon leading to parent-of-origin-specific gene expression, has independently evolved in the endosperm of flowering plants and the placenta of mammals-tissues crucial for nurturing embryos. While transposable elements (TEs) frequently colocalize with imprinted genes and are implicated in imprinting establishment, direct investigations of the impact of de novo TE transposition on genomic imprinting remain scarce. In this study, we explored the effects of chemically induced transposition of the Copia element ONSEN on genomic imprinting in Arabidopsis thaliana. Through the combination of chemical TE mobilization and doubled haploid induction, we generated a line with 40 new ONSEN copies. Our findings reveal a preferential targeting of maternally expressed genes (MEGs) for transposition, aligning with the colocalization of H2A.Z and H3K27me3 in MEGs-both previously identified as promoters of ONSEN insertions. Additionally, we demonstrate that chemically-induced DNA hypomethylation induces global transcriptional deregulation in the endosperm, leading to the breakdown of MEG imprinting. This study provides insights into the consequences of chemically induced TE remobilization in the endosperm, revealing that chemically-induced epigenome changes can have long-term consequences on imprinted gene expression.

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