Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq of DRG-derived cell culture

ABSTRACT: We performed mRNA-seq to characterize cultured dorsal root ganglia (DRG) cells from adult mice. Our data is based on ~25 million reads per sample in six independent RNA preparations. The transcript per millions (TPM) gene count distribution was homogenous between individual cultures. We analyzed the transcriptome for marker genes of different cell types present in the DRG. Expression profiles pointed to a sensory progenitor-like cell type originating from peripheral glial cells of the adult DRG. Such progenitor cells are interesting for reprogramming.

ORGANISM(S): Mus musculus

PROVIDER: GSE261550 | GEO | 2024/06/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Single-cell RNAseq of Neurog1-Neurog2-DsRed transduced DRG cells

Project description:Cultured adult mouse dorsal root ganglia (DRG) cells exhibit glial sensory progenitor properties in vitro. Therefore, they might be a good starter cell for reprogramming into sensory neurons. Here, we infected them with the retroviral vector Neurog2-Neurog1-DsRed to induce sensory neuron development and analyzed by scRNAseq at 14 days post infection whether infected cells show properties of sensory neurons such as nociceptors. After 10x Genomics, data analysis of 4,549 individual cells indicated the generation of neurons, but at an immature cell state.

2024-06-20 | GSE261529 | GEO

Single cell RNAseq Adult Mouse Lumbar Dorsal Root Ganglia (DRG)

Project description:Single cell RNAseq was performed on naïve adult mouse lumbar dorsal root ganglia (DRG) cells. Neuronal and non-neuronal cell populations were identified.

2023-03-07 | GSE198485 | GEO

Anthrax toxins regulate pain signaling and can deliver molecular cargoes to Antxr2+ DRG sensory neurons

Project description:We found that intrathecal injection of anthrax edema toxin (ET), causes analgesia in adult B6J mice. In order to determine if DRG neuron transcriptional responses play a role in pain blockade, we performed a bulk RNA-seq experiment on dissected mouse DRG neurons. We report that anthrax edema toxin causes transcriptional responses in DRG neurons 2h after in vivo administration

2021-09-29 | GSE184619 | GEO

RNA-Seq of DRG and dorsal cord after nerve injury

Project description:Genes are up and down regualted in DRG and spinal dorsal cord after peripheral nerve injury WT male adult with sciatic and femoral nerve transection 7 days, RNA was purified from ipilateral or contralateral L4-L6 DRGs or lumbar spinal dorsal cords

2015-12-10 | E-GEOD-66619 | biostudies-arrayexpress

DRG-CSC co-culture tissue engineered model, CSCs monoculture model and DRG monoculture model

Project description:In this study, we constructed a tissue-engineered DRG-CSC (corneal stromal cells) co-cultured model (DS model), a CSC monoculture model (S model), and a DRG monoculture model (D model). Chicken DRGs were extracted from E7-E10 chicken embryo. Human CSCs were extracted in stromal lenticules from small incision lenticule extraction (SMILE). Transcriptional profiling of DRG in the DS model (DS-D), DRG in the D model(D-D), CSCs in the DS model (DS-S), CSCs in the S model (S-S) was analyzed. After 7 days of culture, RNA of each group was extracted.

2021-06-29 | GSE179020 | GEO

Comparison of wild type and Tmem184b mutant adult DRG

Project description:Comparison of wild type and Tmem184b mutant adult DRG

| PRJNA645875 | ENA

Transcription profiling of wild type dorsal root ganglia (DRG) taken from mice 10-12 weeks, DRG taken from NT-4 -/- mice of 4-5 weeks, NT-4 -/- mice of 12 weeks

Project description:Gene expression profiling was carried out to compare labeled cRNA derived from 3 experimental groups. Group 1 was mRNA extracted from wild type dorsal root ganglia (DRG) taken from mice 10-12 weeks of age, Group 2 was mRNA extracted from DRG taken from NT-4 -/- mice of 4-5 weeks of age. Group 3 was mRNA extracted from DRGs taken from NT-4 -/- mice of 12 weeks of age. In all cases mRNA was extracted from 6-10 mice per group. The experiment was carried out a total of three times.

2012-04-19 | E-MEXP-10 | biostudies-arrayexpress

Conditioning lesion rat DRG time course

Project description:To identify potential regeneration-associated genes, rat dorsal root ganglia (DRG) were lesioned and then sampled at 4, 8, 12, 24, 48, 148, or 296 hrs A total of 23 samples were examined in 8 time points with 2-3 replicates each. For each array, lesioned and uninjured DRG samples were run in a two-color experiment. ----------------------------------------------------- submitters of this record cannot locate the raw data

2015-07-06 | E-GEOD-69466 | biostudies-arrayexpress

Mouse DRG RNA-Seq

Project description:Multiple myeloma (MM) is a neoplasia of B plasma cells that often induces bone pain. However, the mechanisms underlying myeloma-induced bone (MIBP) pain are mostly unknown. Using a localized MM mouse model, we investigated MM induced gene expression changes in the dorsal root ganglia (DRG) innervating the MM-bearing bone and found alterations in pathways associated with cell cycle, immune response and neuronal signalling. The MM transcriptional signature was consistent with metastatic MM infiltration to the DRG, a never-before described feature of the disease that we further demonstrated histologically. In the DRG, MM cells caused loss of vascularization and neuronal injury, which may contribute to late-stage MIBP.

2023-06-26 | GSE216802 | GEO

Expression profiling of DRG from L5 spinal nerve ligation model of neuropathic pain in the rat

Project description:DRG samples were extracted at 28 and 50 days from L5 spinal nerve ligated rats and sham operated rats. Samples were pooled for each time point per group and hybridised to duplicate to RG_u34 genchips (A, B and C chips). Keywords: Disease state analysis

2005-07-07 | GSE2884 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data