Project description:RNA-seq analysis was used to identify the gene expression changes in male and hermaphroditie when daf-18 is lost. Based on these sequencing data, the genes that were up-regulated or down-regulated by at least 2 times (|log2 (folding change) | ≥ 1 and padj ≤ 0.05) were analyzed. The analysis of these differentially expressed genes' pathway and gene ontology (GO) enrichment shows that the influence of different genders after daf-18 deletion is enriched to some signal pathways, including longevity and protein degradation pathway in endoplasmic reticulum, etc.
Project description:RNA-seq analysis was used to identify the gene expression changes of young adults after feeding 5mM tryptophan for 36 hours from the L4 period. Based on these sequencing data, the genes that were up-regulated or down-regulated by at least 2 times (|log2 (folding change) | ≥ 1 and padj ≤ 0.05) were analyzed. The analysis of these differentially expressed genes' pathway and gene ontology (GO) enrichment shows that the influence of different genders after feeding 5mM tryptophan is enriched to some signal pathways, including longevity、ribosome and protein degradation pathway in endoplasmic reticulum, etc.
