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Distinct polyadenylation isoform expression pattherns distinguish between adult urothelia cells across the differentiation spectrum

ABSTRACT: Alternative cleavage and polyadenylation is a major mechanism by which cells diversify their transcriptome to achieve cell state-specific and tissue-specific gene expression patterns. However, mapping polyadenylation isoforms in vivo has been limited to cell types that are easily enriched and purified, such as blood cells, for bulk 3' end sequencing techniques that require significant starting material. We overcame this challenge by repurposing single cell RNA-seq generated from 3' end library preparation to detect and quantify polyadenylation site usage in a cell state-specific manner in the urothelium of adult human ureters.

ORGANISM(S): Homo sapiens

PROVIDER: GSE262863 | GEO | 2025/03/31

REPOSITORIES: GEO

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