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RNA binding properties of NONO and NONO-delta-ER in testis;

ABSTRACT: To investigate the RNA binding proterties of NONO and NONO-delta-ER in testis; we collected adult NONO and NONO-delta-ER mice testis We then performed eCLIP-seq respectively and convey subsequent bioinformatic analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE263329 | GEO | 2024/08/08

REPOSITORIES: GEO

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RNA binding properties of PDHA2, TEX30 in testis; RNA binding properties of HA-NONO and HA-NONO△ER in 3T3L1 cell line.

Project description:To investigate the RNA binding proterties of PDHA2, TEX30 in testis; RNA binding properties of HA-NONO and HA-NONO△ER in 3T3L1 cell line, we collected wildtype testis and HA-NONO/HA-NONO△ER overexpressed 3T3L1 cells. We then performed eCLIP-seq respectively and convey subsequent bioinformatic analysis.

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NONO mutations cause syndromic intellectual disability and inhibitory synaptic defects

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Project description:A large swath of the human proteome is dedicated to mRNA homeostasis, but most RNA-binding proteins lack chemical probes. Here, we report the discovery through phenotypic screening of electrophilic small molecules that swiftly (within 4 h) and stereospecifically decrease transcripts encoding the androgen receptor (AR) and its major V7 splice variant in human prostate cancer cells. We show by chemical proteomics that these compounds covalently engage cysteine-145 of the RNA-binding protein NONO. Broader profiling revealed that covalent NONO ligands suppress a discrete set of transcripts and proteins, including multiple oncogenic transcription factors, and impair the proliferation of cancer cells. These effects were not observed following genetic disruption of NONO, which instead blocked ligand activity. The covalent ligands promote accumulation of NONO in nuclear foci and at the first 5’ splice site of immature transcripts, pointing to a trapping mechanism that may prevent the compensatory action of the paralogous proteins PSPC1 and SFPQ, which were found to increase in cancer cells following genetic or chemical perturbation of NONO. These findings, taken together, designate NONO as a druggable RNA-binding protein that can be co-opted by covalent small molecules to suppress pro-tumorigenic transcriptional networks.

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Diurnal liver transcriptome in wt and nono-/- mice

Project description:The liver circadian transcriptome results from the combined action of a circadian clock and feeding/fasting rhythms. Since we found that (1) the number of NONO-containing speckles increases in response to feeding, (2) NONO interacts with RNA processing factors and (3) it binds to the introns of a large number of protein coding genes, we hypothesized that NONO may contribute to the daily rhythm of hepatic gene expression. To address whether NONO contributes to rhythmic gene expression in response to feeding/fasting, diurnal liver transcriptomes were assessed in nono-/- and wt littermate mice fed a normal diet during the night time (ZT12-24). We habituated mice to a 12:12 LD cycle with food available only at lights off (ZT12-24) for one week and in the second week collected mice every 2h throughout the 24h day. Both total and nuclear RNAs were sequenced after ribosomal RNA depletion

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2024-01-26 | PXD032087 | Pride

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2019-11-01 | GSE133423 | GEO

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