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Functional redundancy of the three insulin receptors of cockroaches

ABSTRACT: Gene duplication is a fundamental evolutionary process which allows to provide opportunities to acquire new gene functions. In the case of the insulin receptor (InR) gene in cockroaches and close-related insects, two successive duplications determined the occurrence of three InR genes: InR2, InR1 and InR3, the last two forming a sister cluster of InR2. The analysis of the sequences in different species and groups did not led to detect positive selection in the divergence of InR1 and InR3. Using the cockroach Blattella germanica as a model, we have determined that InR2 is the gene with the highest expression levels in all the tissues analyzed, both in adult females and males, as well as in nymphs and embryos. InR3 is second in expression levels while InR1 is expressed at lower levels and only in some tissues. The selective depletion by RNAi of each of the three InRs, analyzed in terms of phenotype and fat body transcriptomes, produced essentially redundant effects, with a magnitude approximately proportional to the level of expression of the respective InR. Therefore, the results indicate that the InR duplicates experienced a subfunctionalization process, by which the three InRs maintained similar functions but contributing to those functions proportionally to their expression levels.

ORGANISM(S): Blattella germanica

PROVIDER: GSE263519 | GEO | 2025/01/28

REPOSITORIES: GEO

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