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Hepatic gene expression profile in hydrogen water administrated SD rat

ABSTRACT: The effects of the administration of molecular hydrogen-saturated drinking water (hydrogen water) on hepatic gene expression were investigated in rats. Using DNA microarrays, 548 upregulated and 695 downregulated genes were detected in the liver after a 4-week administration of hydrogen water. Gene Ontology analysis revealed that genes for oxidoreduction-related proteins, including hydroxymethylglutaryl CoA reductase, were significantly enriched in the upregulated genes.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE26363 | GEO | 2010/12/31

SECONDARY ACCESSION(S): PRJNA136881

REPOSITORIES: GEO

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Hepatic gene expression profile in hydrogen water administrated SD rat

Project description:The effects of the administration of molecular hydrogen-saturated drinking water (hydrogen water) on hepatic gene expression were investigated in rats. Using DNA microarrays, 548 upregulated and 695 downregulated genes were detected in the liver after a 4-week administration of hydrogen water. Gene Ontology analysis revealed that genes for oxidoreduction-related proteins, including hydroxymethylglutaryl CoA reductase, were significantly enriched in the upregulated genes. 4-week-old male Sprague-Dawley rats were acclimatized for 4 days in a room maintained at 24 ± 3°C with a relative humidity of 55 ± 15% with a 12-h light-dark cycle (lights on at 7:00, off at 19:00) and were fed ad libitum on a commercial diet (MF, Oriental Yeast Co., Yokohama, Japan). After acclimatization, the rats were divided into two groups (n = 8 per group) with similar mean body weights. During a period of 4 weeks, one group (control group) was supplied with sterilized distilled water and the other (HW group) with hydrogen water produced by MiZ Co. Ltd. (Fujisawa, Japan), which contains 0.7 mM dissolved hydrogen (pH 7.4). All the animals drank water ad libitum. After 4 weeks, each rat was anesthetized with diethyl ether and blood samples were collected from the abdominal aorta. The liver was then excised and analyzed the effect of hydrogen water extract administration on hepatic gene expression.

2010-12-31 | E-GEOD-26363 | biostudies-arrayexpress

Gene expression profiling in mouse livers after administration of both hydrogen-rich water and hydrogen gas

Project description:Molecular hydrogen is a hopeful agent for oxidative stress-related and/or inflammatory disorders. However, the molecular mechanism for these therapeutic effects of hydrogen still remains to be fully elucidated. We examined whether molecular hydrogen alters gene expression levels in normal mouse livers by DNA microarray analysis. We identified 140 mouse genes that were upregulated (31 genes) or downregulated (109 genes) after three weeks of the inhalation of 2% hydrogen-containing air with oral intake of hydrogen-rich water. Ingenuity Pathway Analysis revealed that hydrogen influenced expression of NF-kB- and NFAT-regulated genes. Western blot analysis showed that hydrogen attenuated Erk, p38 MAPK, and NF-kB signaling in mouse livers. We divided 10-week male BALB/c mice into two groups: 1) control group: fed with control water in air (n=4), 2) hydrogen-treated group: fed with hydrogen-rich water in 2% hydrogen/98% air (n=4). Hydrogen-rich water (0.7mM dissolved hydrogen) was generated from distilled water with 0.44 mM Na2SO4 using Aquela Blue, a water-electrolyzing device to produce electrolyzed hydrogen-saturated water near neutral pH (MiZ Co., Ltd, Fujisawa, Japan). The control water was prepared by gently stirring hydrogen-rich water in open air for 24 hours. Hydrogen-rich water or control water was administrated ad libitum to the mice with a 50-ml closed glass vessel equipped with an outlet line having a ball bearing. After 3-week rearing, the mice were sacrificed and their livers were removed for RNA extraction. We mixed equal amount of RNA from four mice in each group (control vs. hydrogen-treated) and compared gene expression profile by microarray.

2014-08-01 | E-GEOD-48826 | biostudies-arrayexpress

Gene expression profiling in mouse livers after administration of both hydrogen-rich water and hydrogen gas

2014-08-01 | GSE48826 | GEO

Expression data from mice liver drinking Hydrogen water

Project description:Liver RNA samples from C57BL6 mice drinking Hydrogen water for 4 weeks We used microarrays to detail the gene expression after drinking hydrogen water.

2017-09-19 | GSE103941 | GEO

Molecular hydrogen stimulates the gene expression of a translational coactivator PGC-1alpha to enhance fatty acid metabolism

Project description:We previously reported that molecular hydrogen (H2) acts as a novel antioxidant to exhibits multiple-functions. Moreover, long-term drinking of H2-water (water infused with H2) enhanced energy expenditure to improve obesity and diabetes in db/db mice, accompanied with the increased gene expression of an energy metabolism stimulatory hepatic hormone, fibroblast growth factor 21 (FGF21); however, the molecular mechanism is unknown particularly on whether this gene regulation is a primary cause or the secondary consequence. Pathway analyses based on comprehensive gene expression revealed the increased expression in various genes involved in fatty acid and steroid metabolisms. As a transcription pathway, the PPARa signaling pathway was identified to up-regulate the genes by 14 day-ingestion of H2. As an early event, the gene expression of PGC-1a was transiently increased at day 3, followed by increased expression of FGF21. In wild-type mice that fed with fatty-diet, H2-water improved the level of plasma triglyceride and extended their life span. H2 was ingested by ad libitum drinking H2-water for 14 days or by oral administration of an H2-producing material MgH2 for 1, 3 or 7 days. Comprehensive gene expression profile in liver of db/db mice was analyzed by DNA microarray.

2015-08-06 | E-GEOD-71738 | biostudies-arrayexpress

Transcription profiling of yeast grown in SD media with addition of either methionine or hydrogen peroxide

Project description:Yeast cells were grown up in SD media containing all required amino acids. Each strain set was performed in triplicate. One set had no changes, the second set had 1mM methionine supplenting the media for the duration of growth and the third set was exposed to 0.5mM hydrogen peroxide for 15 minutes prior to harvesting

2007-05-01 | E-MEXP-998 | biostudies-arrayexpress

Methanococcus maripaludis response to hydrogen limitation

Project description:Methanococcus maripaludis is a methanogenic Archaea that conserves energy from molecular hydrogen to reduce carbon dioxide to methane. Chemostat grown cultures limited for hydrogen, phosphate, or leucine were compared to determine the regulatory response to hydrogen limitation. This was done by comparing hydrogen limited cultures to both leucine limited and phosphate limited cultures. Slow and rapid growing samples limited for either hydrogen or phosphate were compared to determine the regulatory effects of growth rate. Keywords: archaea, hydrogen, leucine, phosphate, nutrient limitation, growth rate, methanogen

2007-04-01 | GSE6747 | GEO

Expression in lymphoblastoid cell lines following hydrogen peroxide bolus at 5 times

Project description:The general expression profile in human lymphoblastoid cell lines treated with 9 uM, 90 uM or 900 uM bolus of hydrogen peroxide at 0, 4,12,24, and 48 hours. Four cell lines (GM07055, GM14569, GM14467, and 12057) were treated separately and total RNA was pooled in equal amounts prior to labeling and hybridization to Affymetrix Human Gene 1.0 ST microarray. Four lymphoblastoid cell lines (GM07055, GM12057, GM14569, and GM14467) were obtained from the Coriell Institute for Medical Research (Camden, NJ). 24 hours before bolus treatments, cells were cultured at a density of 1 x10^6 cells/mL to create 17 cultures for each cell line (a total of 68 samples). At the beginning of the experiment, one culture was collected from each cell line and total RNA prepared, this was equally pooled and prepared for microarray analysis (time zero, treatment zero). Each cell line was then treated with a 9 (4 cultures), 90 (4 cultures), or 900 uM hydrogen peroxide bolus (4 cultures) or water (4 cultures). Four, 12, 24 or 48 hours following the hydrogen peroxide bolus, cells were collected for each cell line of each treatment and total RNA prepared. RNA from the four cell lines were equalling pooled for each time and each hydrogen peroxide (or water) bolus amount resulting in a total of 16 additional samples which were also labeled and hybridized to Affymetrix Human Gene 1.0 ST microarrays.

2010-07-30 | E-GEOD-21800 | biostudies-arrayexpress

Expression data from mice liver drinking Hydrogen water

Project description:Expression data from mice liver drinking Hydrogen water

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relationship between the level of plasma hydrogen sulfide in the patients with colorectal cancer

Project description:Interventions: Case series:nill;The control group:nill Primary outcome(s): Hydrogen sulfide;Pathological diagnosis Study Design: Case-Control study

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