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Microarray analysis of CaSki cell line grown as spheroids and adherent conditions

ABSTRACT: Microarry based whole genome expression analysis was done for the more tumorigenic CaSki spheroids cultures and the less tumorigenic CaSki adherent cultures to identify signaling mechanisms that vary between the two populations. Subsequent to this, an independent array was done to characterize the differences in gene expression between the cancer stem cell-like Lin-CD66+ and the bulk Lin- CD66- cells isolated from primary human cervical carcinomas. The second array was custom-designed and had genes implicated in stemness, epithelial differentiation, signaling pathways implicated in stemness (Notch and Wnt) and cell cylce-regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE26417 | GEO | 2011/06/09

SECONDARY ACCESSION(S): PRJNA142251

REPOSITORIES: GEO

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Microarray analysis of CaSki cell line grown as spheroids and adherent conditions

Project description:Microarry based whole genome expression analysis was done for the more tumorigenic CaSki spheroids cultures and the less tumorigenic CaSki adherent cultures to identify signaling mechanisms that vary between the two populations. Subsequent to this, an independent array was done to characterize the differences in gene expression between the cancer stem cell-like Lin-CD66+ and the bulk Lin- CD66- cells isolated from primary human cervical carcinomas. The second array was custom-designed and had genes implicated in stemness, epithelial differentiation, signaling pathways implicated in stemness (Notch and Wnt) and cell cylce-regulation. Microarray analysis of CaSki cell line grown as spheroids and adherent conditions

2011-06-09 | E-GEOD-26417 | biostudies-arrayexpress

Microarray analysis of Lin- CD66+ and Lin- CD66- cells isolated from primary human cervical cancer

Project description:Microarry based whole genome expression analysis was done for the more tumorigenic CaSki spheroids cultures and the less tumorigenic CaSki adherent cultures to identify signaling mechanisms that vary between the two populations. Subsequent to this, an independent array was done to characterize the differences in gene expression between the cancer stem cell-like Lin-CD66+ and the bulk Lin- CD66- cells isolated from primary human cervical carcinomas. The second array was custom-designed and had genes implicated in stemness, epithelial differentiation, signaling pathways implicated in stemness (Notch and Wnt) and cell cylce-regulation. Microarray analysis of Lin- CD66+ and Lin- CD66- cells isolated from primary human cervical cancer

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Microarray analysis of Lin- CD66+ and Lin- CD66- cells isolated from primary human cervical cancer

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Project description:Microarry based whole genome expression analysis was done for the more tumorigenic CaSki spheroids cultures and the less tumorigenic CaSki adherent cultures to identify signaling mechanisms that vary between the two populations. Subsequent to this, an independent array was done to characterize the differences in gene expression between the Lin-CD66+ and the Lin- CD66- cells isolated from primary human cervical carcinomas. The second array was custom-designed and had genes implicated in stemness, epithelial differentiation, signaling pathways implicated in stemness (Notch and Wnt) and cell cylce-regulation. Refer to individual Series. This SuperSeries is composed of the following subset Series: GSE26417: Microarray analysis of CaSki cell line grown as spheroids and adherent conditions GSE26418: Microarray analysis of Lin- CD66+ and Lin- CD66- cells isolated from primary human cervical cancer

2011-06-09 | E-GEOD-26419 | biostudies-arrayexpress

Notch signaling in CD66+ cells drives the progression of human cervical cancers

Project description:Microarry based whole genome expression analysis was done for the more tumorigenic CaSki spheroids cultures and the less tumorigenic CaSki adherent cultures to identify signaling mechanisms that vary between the two populations. Subsequent to this, an independent array was done to characterize the differences in gene expression between the Lin-CD66+ and the Lin- CD66- cells isolated from primary human cervical carcinomas. The second array was custom-designed and had genes implicated in stemness, epithelial differentiation, signaling pathways implicated in stemness (Notch and Wnt) and cell cylce-regulation. This SuperSeries is composed of the SubSeries listed below.

2011-06-09 | GSE26419 | GEO

CaSki cells: adherent cultured cell vs. sphere cells

Project description:Transcriptional profiling of human cervical squamous cell carcinoma cell line CaSki comparing adherent cultured cells with spheres cultured in serum free culture medium. Goal was to identify candidate antigens for immunotherapy targeting cancer stem cells. Two-condition experiment, CaSki vs. CaSki-sphere cells.

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Homo sapiens

Project description:Microarray analysis of Lin- CD66+ and Lin- CD66- cells isolated from primary human cervical cancer

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RNA-seq analysis of CaSki/SiHa/HeLa cell lines

Project description:We perfomed RNA-seq analysis using HPV16-postive CaSki and SiHa cells and HPV18-postive HeLa cells for HPV integration and gene expression analyses

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CaSki cells: adherent cultured cell vs. sphere cells

2015-03-04 | GSE65499 | GEO

RNA sequencing analysis of DLEU2 knockdown in CaSki cells

Project description:To further explore the molecular mechanism of DLEU2 in CC cells, RNA sequencing analysis was performed to investigate the differential gene expression profiles between DLEU2 knockdown group and comtrol group in CaSki cells.

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