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Tra2b's Ultraconserved Poison Exon relays TCR stimulation to shape T cell fate [N4T4Seq]

ABSTRACT: Analysis of transcript changes with enough depth for evaluation of splicing changes between CD8+ T cells from mice receiving high affinity SIINFEKL (N4) and moderate affinity SIITFEKL (T4) peptide + anti-OX40/anti-41BB costimulation

ORGANISM(S): Mus musculus

PROVIDER: GSE267588 | GEO | 2024/06/01

REPOSITORIES: GEO

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Tra2b's Ultraconserved Poison Exon relays TCR stimulation to shape T cell fate [CLIP-seq]

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Expression data from C57/Bl6 Wt mice CD4+ T cells after activation with anti-CD3 plus Wt APC or OX40L-Tg APC

Project description:OX40 is a T cell costimulatory molecule in the tumor necrosis factor receptor (TNFR) superfamily. Unlike other costimulatory molecules, OX40 is not expressed by naive T cells, but its expression is rapidly induced following T cell activation, and OX40 costimulation plays a critical role in cell survival, proliferation, and generation of memory cells. We used microarrays to detail the global programme of gene expression of activated CD4 T cells underlying OX40 costimulation and identified distinct classes of up-regulated genes after OX40 costimulation.

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Global chromatin accessibility of gene locus in CD4+ T cells activated with or without OX40 costimulation

Project description:OX40 is a T cell costimulatory molecule in the tumor necrosis factor receptor (TNFR) superfamily. Unlike other costimulatory molecules, OX40 is not expressed by naive T cells, but its expression is rapidly induced following T cell activation, and OX40 costimulation plays a critical role in cell survival, proliferation, and generation of memory cells. We used ATAC-seq to detail the chromatin accessibility of gene locus of activated CD4 T cells with or without OX40 costimulation in the presence of TGF-β1 and IL-2 and identified distinct classes of genes after OX40 costimulation.

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