Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

N6-methyladenosine (m6A) modification in HaCaT cells (immortalized human keratinocyte cell line) treated with STM2457 in the presence or absence of M5 (inflammatory factor cocktail) stimulation [MeRIPseq_KC]

ABSTRACT: We conducted an analysis of N6-methyladenosine (m6A) modifications in HaCaT cells, an immortalized human keratinocyte cell line, following treatment with METTL3 inhibitor, STM2457, under conditions of either M5 (inflammatory factor cocktail) stimulation or its absence. The primary aim of this investigation was to establish a molecular framework elucidating the role of m6A modifications in modulating the functional dynamics of keratinocytes within an inflammatory milieu.

ORGANISM(S): Homo sapiens

PROVIDER: GSE268746 | GEO | 2024/08/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Knocking down the eIF4E gene in HaCaT cells, which M5 stimulates to establish the in vitro psoriasis cell model.

Project description:We transfected keratinocytes cell line (HaCaT cells) with si-NC and si-eIF4E, and added M5 into the cell culture medium to reveal the function of eIF4E in the in vitro psoriasis cell model.

2023-11-22 | GSE247463 | GEO

The N6-methyadenosine (m6A) levels in HaCaT-T cells and control HaCaT cells

Project description:Human m6A-mRNA&lncRNA Epitranscriptomic Microarray of arsenite-transformed human keratinocytes (HaCaT-T cells, 1 μM arsenite exposure for 50 passages) compared to its control HaCaT cells (passed for 50 passages without arsenic exposure).

2020-11-08 | GSE161033 | GEO

Whole Genome CGH array on patients with acute myeloid leukemia-M5 (AML-M5)

Project description:Unbalanced chromosomal abnormalities might play a major role in the leukemogenesis of AML-M5 Oligonucelotide array CGH were performed on 24 patients with AML-M5

2013-12-18 | GSE53429 | GEO

Whole Genome CGH array on patients with acute myeloid leukemia-M5 (AML-M5)

Project description:Unbalanced chromosomal abnormalities might play a major role in the leukemogenesis of AML-M5 Oligonucelotide array CGH were performed on 24 patients with AML-M5 To assess the possible existence of unbalanced chromosomal abnormalities and delineate the characterization of copy number alterations (CNAs) of acute myeloid leukemia-M5 (AML-M5)

2013-12-18 | E-GEOD-53429 | biostudies-arrayexpress

Temporal gene regulation of human keratinocyte HaCaT cells treated with obacunone

Project description:Obacunone treatment upregulates gene expression of cholesterol metabolism-associated genes in HaCaT cells Total RNA obtained from HaCaT cells treated with 100 uM obacunone for 0.5, 1, 3, 6, or 12 h was compared to that of vehicle control cells.

2014-04-29 | E-GEOD-57080 | biostudies-arrayexpress

Gene expression profile analysis of human keratinocyte HaCaT cells treated with obacunone

Project description:Obacunone treatment upregulates gene expression of cholesterol and lipid metabolism-associated genes in HaCaT cells Total RNA obtained from HaCaT cells treated with 10, 50, or 100 uM obacunoe for 4 h was compared to that of vehicle control cells.

2014-04-29 | E-GEOD-57120 | biostudies-arrayexpress

Study of transcriptional effects of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line.

Project description:Aim of the study was to characterize at a molecular level (changes in transcriptomes) the effect of monosodium urate crystal (MSU) on HaCaT keratinocyte cell line. This was adressed by using a culture model. The HaCaT cell line (human keratinocytes) was stimulated by MSU (1mg/mL) vs control for 12 hrs. By using genome-wide expression profiling, we identified deregulation of functionally relevant gene networks. HaCaT were obtained from Cell Lines Service (Eppelheim, Germany) and grown in DMEM medium (PAN biotech, Aidenbach, Germany) supplemented with 10% FBS (Life Technology, Grand Island, NY, USA), L-glutamine and non-essential amino acid. Before the treatment HaCaT cells were cultured in serum-free medium for 12hrs. HaCaT were treated with MSU (1mg/ml) vs DMEM control for 12hrs then submitted to RNA extration and gene expression profiling. Triplicate experiments were performed: HaCaT control (n=3), MSU-treated (n=3).

2015-10-29 | E-GEOD-65931 | biostudies-arrayexpress

The miRNA microarray data after UVB exposure to human keratinocyte HaCaT cells

Project description:UVB plays a key role in inflammation and DNA damage in human skin. Human keratinocyte HaCaT cells were utilized to determine the up- and down-regulated miRNA after UVB exposure. We used microarrays to identify the miRNA affected after UVB (15 mJ/cm^2) exposure to HaCaT cells

2020-03-23 | GSE142534 | GEO

Temporal gene regulation of human keratinocyte HaCaT cells treated with obacunone

Project description:Obacunone treatment upregulates gene expression of cholesterol metabolism-associated genes in HaCaT cells

2014-04-29 | GSE57080 | GEO

RNA-seq of C18-4 and TM4 cells samples from different M5 receptor modification groups

Project description:The goat of this project is to explore M5 receptor regulation spermatogenesis. We tried to search the mechanism of M5 receptor regulation spermatogenesis. RNA-seq of C18-4 and TM4 cells samples from different groups: C3NC, M5 knockdown in C18-4, TM3NC, M5 knockdown in TM4 cells. The cell were transfected with virus with inhibition of M5 expression ( M5 gene knockdown).

2021-06-16 | GSE142618 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data