Transcriptomics

Dataset Information

0

GTestimate: Improving relative gene expression estimation in scRNA-seq using the Good-Turing estimator


ABSTRACT: Single-cell RNA-seq suffers from unwanted technical variation between cells, caused by its complex experiments and shallow sequencing depths. We present GTestimate a new normalization method based on the Good-Turing estimator, which improves upon conventional methods by accounting for unobserved genes. We validate GTestimate using new ultra-deep sequencing data, generated via a novel cell targeted PCR-amplification approach, and show substantial improvements in cell-cell distance estimation and downstream results.

ORGANISM(S): Homo sapiens

PROVIDER: GSE268930 | GEO | 2024/07/31

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA1119477 | ENA
| S-EPMC6657110 | biostudies-literature
| S-EPMC6621925 | biostudies-literature
| S-EPMC3709873 | biostudies-literature
| S-EPMC8172148 | biostudies-literature
| S-EPMC4098781 | biostudies-literature
2024-08-30 | GSE275783 | GEO
2024-08-30 | GSE275782 | GEO
| S-EPMC4898800 | biostudies-literature
| S-EPMC3038348 | biostudies-literature