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Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses

ABSTRACT: Cell isolation protocols from brain tissue constitute prolonged ex vivo processing durations, rendering them suboptimal for transcriptomic studies. Εspecially, yielding sufficient quantities of microglia or vascular cells requires the use of large tissue volumes and the addition of an enrichment step. In this study, we developed a simple, rapid, and reproducible cell isolation method for generation of single-cell suspensions from micro-dissected brain regions, providing high numbers of microglia and vascular cells, without requiring an additional encrihment step. Cells isolated using this method are suitable for molecular profiling studies using 10× Genomics Chromium single-cell RNA sequencing with high reproducibility. Our method is valuable for longitudinal unbiased molecular profiling of microglia and vascular cells within different brain regions, spanning multiple time points across physiological development or disease progression.

ORGANISM(S): Mus musculus

PROVIDER: GSE270158 | GEO | 2024/09/30

REPOSITORIES: GEO

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Rapid and robust isolation of microglia and vascular cells from brain subregions for integrative single-cell analyses

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